CPC C12N 5/0636 (2013.01) [A61K 35/17 (2013.01); C12N 2501/21 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/515 (2013.01); C12N 2501/58 (2013.01); C12N 2502/1121 (2013.01); C12N 2510/00 (2013.01)] | 10 Claims |
1. A method of culturing T cells, the method comprising culturing T cells in a culture vessel in the presence of a T cell stimulator, an exogenous CCL21 and an exogenous ICAM1, thereby culturing the T cells, wherein each of said CCL21 and ICAM1 are immobilized to a solid support, wherein said T cell stimulator is an antigen-specific stimulator comprising a dendritic cell loaded with an antigen, wherein said immobilized CCL21 and ICAM1 are attached to said culture vessel, and wherein said T cells comprise engineered T cells transduced with a nucleic acid encoding a T cell receptor (TCR) or a chimeric antigen receptor (CAR).
|