	US 11,932,845 B2
	Microorganisms and methods for production of 4-hydroxybutyrate, 1,4-butanediol and related compounds
Priti Pharkya, San Diego, CA (US); Anthony P. Burgard, Bellefonte, PA (US); Stephen J. Van Dien, Encinitas, CA (US); Robin E. Osterhout, San Diego, CA (US); Mark J. Burk, San Diego, CA (US); John D. Trawick, La Mesa, CA (US); Michael P. Kuchinskas, Escondido, CA (US); and Brian Steer, San Diego, CA (US)
Assigned to Genomatica, Inc., San Diego, CA (US)
Appl. No. 14/405,390
Filed by Genomatica, Inc., San Diego, CA (US)
PCT Filed Jun. 3, 2013, PCT No. PCT/US2013/043954 § 371(c)(1), (2) Date Dec. 3, 2014, PCT Pub. No. WO2013/184602, PCT Pub. Date Dec. 12, 2013.
Claims priority of provisional application 61/655,429, filed on Jun. 4, 2012.
Prior Publication US 2015/0148513 A1, May 28, 2015
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/18 (2006.01); C12P 7/42 (2006.01); C12P 7/52 (2006.01); C12N 1/20 (2006.01); C12P 7/18 (2006.01); C12N 15/52 (2006.01); C12N 1/14 (2006.01); C12N 1/16 (2006.01); C12N 15/63 (2006.01); C07C 31/20 (2006.01); C07C 59/01 (2006.01); C08G 18/28 (2006.01); C08G 18/32 (2006.01); C08G 63/06 (2006.01); C08G 63/16 (2006.01); C08G 63/181 (2006.01); C08G 69/02 (2006.01)

CPC C12N 1/20 (2013.01) [C07C 31/207 (2013.01); C07C 59/01 (2013.01); C08G 18/284 (2013.01); C08G 18/3206 (2013.01); C08G 63/06 (2013.01); C08G 63/16 (2013.01); C08G 63/181 (2013.01); C08G 69/02 (2013.01); C12N 1/14 (2013.01); C12N 1/16 (2013.01); C12N 9/18 (2013.01); C12N 15/52 (2013.01); C12N 15/63 (2013.01); C12P 7/18 (2013.01); C12P 7/42 (2013.01); C12Y 301/01 (2013.01); Y02P 20/52 (2015.11)]

11 Claims

1. A non-naturally occurring microbial organism, said microbial organism having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein said microbial organism comprises a genetic modification that increases expression of a gamma-butyrolactone esterase relative to the microbial organism in the absence of the genetic modification to increase expression of a gamma-butyrolactone esterase, wherein the gamma-butyrolactone esterase converts gamma-butyrolactone to 4-hydroxybutyrate and decreases production of gamma-butyrolactone relative to the microbial organism in the absence of the genetic modification to increase expression of a gamma-butyrolactone esterase, wherein the gamma-butyrolactone esterase is expressed in a sufficient amount to produce 4-hydroxybutyrate, and wherein the microbial organism comprises a genetic modification that decreases expression of an alcohol dehydrogenase relative to the microbial organism in the absence of the genetic modification to decrease expression of an alcohol dehydrogenase.