CPC A61K 39/0258 (2013.01) [A61P 31/04 (2018.01); A61P 37/04 (2018.01); C07K 14/21 (2013.01); C07K 19/00 (2013.01); C12N 9/1051 (2013.01); C12Y 204/01 (2013.01); A61K 2039/575 (2013.01); A61K 2039/6037 (2013.01)] | 22 Claims |
1. A recombinant host cell for producing a bioconjugate of an E. coli glucosylated O4 antigen polysaccharide covalently linked to a carrier protein, wherein the glucosylated O4 antigen polysaccharide comprises the structure of Formula
wherein n is an integer of 5 to 40,
the host cell comprising:
(i) a nucleotide sequence of an rfb gene cluster for the E. coli O4 antigen polysaccharide;
(ii) a nucleotide sequence encoding a glucosyl transferase, wherein the glucosyl transferase is capable of modifying the E. coli O4 antigen polysaccharide to produce the E. coli glucosylated O4 antigen polysaccharide;
(iii) a nucleotide sequence encoding a translocase that is capable of translocating bactoprenol-linked glucose;
(iv) a nucleotide sequence encoding a glycosyltransferase that is capable of glucosylating bactoprenol;
(v) a nucleotide sequence encoding the carrier protein; and
(vi) a nucleotide sequence encoding an oligosaccharyl transferase capable of covalently linking the E. coli glucosylated O4 antigen polysaccharide to the carrier protein to produce the bioconjugate.
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