US 11,931,400 B2
Therapeutic and diagnostics compositions targeting toll-like receptors and methods thereof
Zachary Apte, San Francisco, CA (US); Jessica Richman, San Francisco, CA (US); Daniel Almonacid, San Francisco, CA (US); Melissa Alegria, San Francisco, CA (US); Valeria Marquez, San Francisco, CA (US); Eduardo Morales, San Francisco, CA (US); Rodrigo Ortiz, San Francisco, CA (US); Janet Torres, San Francisco, CA (US); Paulo Covarrubias, San Francisco, CA (US); Javier Gimpel, San Francisco, CA (US); and Ingrid Araya, San Francisco, CA (US)
Assigned to PSOMAGEN, INC., Rockville, MD (US)
Filed by PSOMAGEN, INC., Rockville, MD (US)
Filed on Mar. 7, 2018, as Appl. No. 15/914,925.
Claims priority of provisional application 62/536,924, filed on Jul. 25, 2017.
Claims priority of provisional application 62/468,286, filed on Mar. 7, 2017.
Prior Publication US 2018/0311310 A1, Nov. 1, 2018
Int. Cl. C07K 14/00 (2006.01); A61K 38/08 (2019.01); A61K 38/16 (2006.01); G01N 33/68 (2006.01)
CPC A61K 38/164 (2013.01) [A61K 38/08 (2013.01); C07K 14/001 (2013.01); G01N 33/68 (2013.01); Y02A 50/30 (2018.01)] 12 Claims
 
1. A therapeutic composition for improving a Toll-like receptor (TLR)-related condition, the therapeutic composition comprising a peptide configured to modulate TLR-related functionality and derived based on reengineering of a target peptide sequence associated with a microorganism-related modulator associated with Amuc_1100 protein from Akkermansia muciniphila, wherein the peptide corresponds to a peptide sequence comprising at least one motif of a set of peptide sequence motifs comprising:
a first motif comprising XXGFXLKXX (SEQ ID NO: 1), wherein X in the first motif comprises any amino acid type or no amino acid;
a second motif comprising XXTPA[Z][B]XXX (SEQ ID NOS:2-5), wherein X in the second motif comprises any amino acid type or no amino acid, wherein Z in the second motif comprises at least one of (N,D), and wherein B in the second motif comprises at least one of (N,E);
a third motif comprising [X][Z][B]APW (SEQ ID NOS:6-245), wherein X in the third motif comprises at least one of (I,F,W,Y,A), wherein Z in the third motif comprises at least one of (D,A,Q,E,G,H,I,L,F,P,T,Y), and wherein B in the third motif comprises at least one of (Q,L,W,Y);
a fourth motif comprising PP[X][Z][B][O]P (SEQ ID NOS:246-693), wherein X in the fourth motif comprises at least one of (Y,P), wherein Z in the fourth motif comprises at least one of (W,Y,F,A,V,C,T,L,S,H,Q,E,G,I), wherein B in the fourth motif comprises at least one of (W,I,F,P,D,G,C,A), and wherein O in the fourth motif comprises at least one of (G,N);
a fifth motif comprising [X][Z][i][O][B][J][f] (SEQ ID NOS: 694-35253), wherein X in the fifth motif comprises at least one of (G,Y,W), wherein Z in the fifth motif comprises at least one of (W,V,I,F,R,M,S,L), wherein i in the fifth motif comprises at least one of (I,G), wherein O in the fifth motif comprises at least one of (Y,P,H,L,F), wherein B in the fifth motif comprises at least one of (I,D,Y,F,V,T,G,A,Q), wherein J in the fifth motif comprises at least one of (F,R,C,L,A,Q,D,V), and wherein fin the fifth motif comprises at least one of (F,Y);
a tenth motif comprising [X][J]TAYKP[B] (SEQ ID NOS:35304-35323), wherein X in the tenth motif comprises at least one of (W,L), wherein J in the tenth motif comprises at least one of (A,N,S,W,E), and wherein B in the tenth motif comprises at least one of (S,F);
an eleventh motif comprising [X]A[J][B]AP[Z]I[O][U] (SEQ ID NOS:35324-35803), wherein X in the eleventh motif comprises at least one of (N,W,A), wherein J in the eleventh motif comprises at least one of (F,N,Y,P), wherein B in the eleventh motif comprises at least one of (P,H,Y,W,A), wherein Z in the eleventh motif comprises at least one of (Y,A), wherein O in the eleventh motif comprises at least one of (W,Q), and wherein U in the eleventh motif comprises at least one of (R, Q); and
a twelfth motif comprising H[X][J]QP[B]AQEP (SEQ ID NOS:35804-35839), wherein X in the twelfth motif comprises at least one of (E,G,W,F), wherein J in the twelfth motif comprises at least one of (G,A,N), and wherein B in the twelfth motif comprises at least one of (H,W,K).