Yifeng Yin, Elk Grove, CA (US); Michael Schnall-Levin, San Francisco, CA (US); and Eswar Prasad Ramachandran Iyer, Oakland, CA (US)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Mar. 16, 2023, as Appl. No. 18/185,118.
Application 18/185,118 is a continuation of application No. 17/212,681, filed on Mar. 25, 2021, granted, now 11,649,485.
Application 17/212,681 is a continuation of application No. PCT/US2019/065013, filed on Dec. 6, 2019.
Claims priority of provisional application 62/941,581, filed on Nov. 27, 2019.
Claims priority of provisional application 62/939,488, filed on Nov. 22, 2019.
Claims priority of provisional application 62/935,043, filed on Nov. 13, 2019.
Claims priority of provisional application 62/934,883, filed on Nov. 13, 2019.
Claims priority of provisional application 62/931,587, filed on Nov. 6, 2019.
Claims priority of provisional application 62/931,779, filed on Nov. 6, 2019.
Claims priority of provisional application 62/860,993, filed on Jun. 13, 2019.
Claims priority of provisional application 62/842,463, filed on May 2, 2019.
Claims priority of provisional application 62/839,264, filed on Apr. 26, 2019.
Claims priority of provisional application 62/839,575, filed on Apr. 26, 2019.
Claims priority of provisional application 62/839,212, filed on Apr. 26, 2019.
Claims priority of provisional application 62/839,346, filed on Apr. 26, 2019.
Claims priority of provisional application 62/839,320, filed on Apr. 26, 2019.
Claims priority of provisional application 62/839,223, filed on Apr. 26, 2019.
Claims priority of provisional application 62/822,722, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,680, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,606, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,605, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,554, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,566, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,627, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,592, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,565, filed on Mar. 22, 2019.
Claims priority of provisional application 62/822,610, filed on Mar. 22, 2019.
Claims priority of provisional application 62/819,449, filed on Mar. 15, 2019.
Claims priority of provisional application 62/819,478, filed on Mar. 15, 2019.
Claims priority of provisional application 62/819,456, filed on Mar. 15, 2019.
Claims priority of provisional application 62/819,495, filed on Mar. 15, 2019.
Claims priority of provisional application 62/812,219, filed on Feb. 28, 2019.
Claims priority of provisional application 62/811,495, filed on Feb. 27, 2019.
Claims priority of provisional application 62/788,885, filed on Jan. 6, 2019.
Claims priority of provisional application 62/788,897, filed on Jan. 6, 2019.
Claims priority of provisional application 62/788,871, filed on Jan. 6, 2019.
Claims priority of provisional application 62/788,906, filed on Jan. 6, 2019.
Prior Publication US 2023/0323434 A1, Oct. 12, 2023
Int. Cl. C12Q 1/6837 (2018.01)
CPC C12Q 1/6837 (2013.01)
29 Claims
1. A method of generating a spatial array comprising:
(a) immobilizing a first plurality of primers and a second plurality of primers on a substrate, wherein:
(i) a first primer of the first plurality of primers comprises one or more functional domains and a sequence complementary to a hybridization domain, wherein the one or more functional domains of the first primer comprise a sequencing specific site or a primer binding site; and
(ii) a second primer of the second plurality of primers comprises a sequence complementary to a capture domain;
(b) providing a template oligonucleotide comprising the hybridization domain capable of hybridizing to the sequence complementary to the hybridization domain of the first primer, a complement of a spatial barcode, and a complement of the capture domain;
(c) hybridizing the hybridization domain of the template oligonucleotide to the sequence complementary to the hybridization domain of the first primer;
(d) extending the first primer using the template oligonucleotide as a template to generate a capture probe comprising the first primer, the spatial barcode, and the capture domain; and
(e) releasing the template oligonucleotide from the capture probe, thereby generating the spatial array.