US 11,926,865 B2
On-slide staining by primer extension
Nikolay Samusik, Jacksonville, OR (US); Garry P. Nolan, Redwood City, CA (US); and Yury Goltsev, Stanford, CA (US)
Assigned to THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on Feb. 15, 2022, as Appl. No. 17/672,552.
Application 17/672,552 is a continuation of application No. 16/240,463, filed on Jan. 4, 2019, granted, now 11,299,770.
Application 16/240,463 is a continuation of application No. 15/986,594, filed on May 22, 2018, abandoned.
Application 15/986,594 is a continuation of application No. 15/832,595, filed on Dec. 5, 2017, granted, now 10,017,808, issued on Jul. 10, 2018.
Application 15/832,595 is a continuation of application No. 14/560,921, filed on Dec. 4, 2014, granted, now 9,909,167, issued on Mar. 6, 2018.
Claims priority of provisional application 62/015,799, filed on Jun. 23, 2014.
Prior Publication US 2023/0002809 A1, Jan. 5, 2023
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6804 (2018.01); C12Q 1/6818 (2018.01)
CPC C12Q 1/6818 (2013.01) [C12Q 1/6804 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A composition comprising:
(a) a plurality of capture agents, wherein the plurality of capture agents can specifically bind to different complementary sites in a biological sample,
wherein each of the plurality of capture agents comprises a double stranded nucleic acid, and wherein a 3′ end of a first strand of the double stranded nucleic acid is recessed relative to a 5′ end of a second strand of the double stranded nucleic acid, thereby producing a 5′ overhang;
(b) a polymerase;
(c) a plurality of nucleotides;
wherein the polymerase is configured to extend a strand of the double stranded nucleic acid with the plurality of nucleotides; and
wherein:
(i) the plurality of nucleotides comprise a fluorescent nucleotide and/or
(ii) the composition further comprises a fluorescently labeled oligonucleotide complementary to a sequence of an overhang of at least one capture agent of the plurality of capture agents.