Lynne Gilson, Encinitas, CA (US); Steven J. Collier, Concord, MA (US); Joly Sukumaran, Singapore (SG); Wan Lin Yeo, Singapore (SG); Oscar Alvizo, Fremont, CA (US); Ee Ling Teo, Mauchline East Ayr (GB); Robert J. Wilson, San Francisco, CA (US); and Junye Xu, Singapore (SG)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on May 25, 2021, as Appl. No. 17/330,172.
Application 15/899,907 is a division of application No. 15/372,788, filed on Dec. 8, 2016, granted, now 9,932,616, issued on Apr. 3, 2018.
Application 14/710,893 is a division of application No. 14/465,467, filed on Aug. 21, 2014, granted, now 9,181,570, issued on Nov. 10, 2015.
Application 14/465,467 is a division of application No. 13/755,113, filed on Jan. 31, 2013, granted, now 8,846,362, issued on Sep. 30, 2014.
Application 13/755,113 is a division of application No. 12/890,134, filed on Sep. 24, 2010, granted, now 8,383,382, issued on Feb. 26, 2013.
Application 17/330,172 is a continuation of application No. 16/784,011, filed on Feb. 6, 2020, granted, now 11,046,982.
Application 16/784,011 is a continuation of application No. 15/899,907, filed on Feb. 20, 2018, granted, now 10,590,445, issued on Mar. 17, 2020.
Application 15/372,788 is a continuation of application No. 14/710,893, filed on May 13, 2015, granted, now 9,546,388, issued on Jan. 17, 2017.
Claims priority of provisional application 61/247,274, filed on Sep. 30, 2009.
Claims priority of provisional application 61/247,253, filed on Sep. 30, 2009.
Prior Publication US 2021/0277432 A1, Sep. 9, 2021
1. An engineered variant LovD polypeptide having at least two-fold greater catalytic activity than the wild-type Aspergillus terreus acyltransferase of the amino acid sequence of SEQ ID NO: 2, wherein said engineered variant LovD polypeptide has at least 90% sequence identity to the wild-type A. terreus acyltransferase of the amino acid sequence of SEQ ID NO: 2, and wherein said engineered variant LovD polypeptide comprises the substitution.