US 11,926,856 B2
Method for producing D-psicose
Xinyu Shen, Wake Forest, NC (US); Randall Scott Deinhammer, Wake Forest, NC (US); James Ron Huffman, Wake Forest, NC (US); Kendra Stallings, Wake Forest, NC (US); Tine Hoff, Holte (DK); Jesper Salomon, Holte (DK); and Anne Goldbech Olsen, Valby (DK)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Filed by Novozymes A/S, Bagsvaerd (DK)
Filed on Jun. 2, 2022, as Appl. No. 17/830,420.
Application 17/830,420 is a division of application No. 16/638,191, granted, now 11,377,650, previously published as PCT/EP2018/073317, filed on Aug. 30, 2018.
Claims priority of provisional application 62/552,746, filed on Aug. 31, 2017.
Prior Publication US 2022/0315912 A1, Oct. 6, 2022
Int. Cl. C12N 9/90 (2006.01); C12P 19/02 (2006.01); C12P 19/24 (2006.01)
CPC C12N 9/90 (2013.01) [C12P 19/02 (2013.01); C12P 19/24 (2013.01); C12Y 501/03 (2013.01)] 14 Claims
 
1. A method for producing D-psicose, the method comprising: step (a) contacting a composition comprising a polypeptide having D-psicose 3-epimerase activity with D-fructose under conditions suitable for the polypeptide having D-psicose 3-epimerase activity to convert D-fructose to D-psicose; and optionally step (b) recovering the produced D-psicose, wherein the polypeptide having D-psicose 3-epimerase activity is selected from the group consisting of:
(i) a polypeptide having an amino acid sequence comprising at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4:
(ii) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3; and
(iii) a fragment of the polypeptide of SEQ ID NO: 2 having at least 90% of the amino acid residues of the polypeptide of SEQ ID NO: 2 that has D-psicose 3-epimerase activity, or a fragment of the polypeptide of SEQ ID NO: 4 having at least 90% of the amino acid residues of SEQ ID NO: 4 that has D-psicose 3-epimerase activity.