CPC C12N 9/90 (2013.01) [C12P 19/02 (2013.01); C12P 19/24 (2013.01); C12Y 501/03 (2013.01)] | 14 Claims |
1. A method for producing D-psicose, the method comprising: step (a) contacting a composition comprising a polypeptide having D-psicose 3-epimerase activity with D-fructose under conditions suitable for the polypeptide having D-psicose 3-epimerase activity to convert D-fructose to D-psicose; and optionally step (b) recovering the produced D-psicose, wherein the polypeptide having D-psicose 3-epimerase activity is selected from the group consisting of:
(i) a polypeptide having an amino acid sequence comprising at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4:
(ii) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3; and
(iii) a fragment of the polypeptide of SEQ ID NO: 2 having at least 90% of the amino acid residues of the polypeptide of SEQ ID NO: 2 that has D-psicose 3-epimerase activity, or a fragment of the polypeptide of SEQ ID NO: 4 having at least 90% of the amino acid residues of SEQ ID NO: 4 that has D-psicose 3-epimerase activity.
|