CPC C12N 9/248 (2013.01) [A23K 10/14 (2016.05); C12N 9/24 (2013.01); C12N 9/2402 (2013.01); C12P 19/14 (2013.01); C12Y 302/01008 (2013.01); C12Y 302/01055 (2013.01); C07K 2319/02 (2013.01); C07K 2319/21 (2013.01); C07K 2319/55 (2013.01)] | 18 Claims |
1. A composition comprising:
a glycoside hydrolase family 10 (GH10) polypeptides having xylanase activity and at least 80% sequence identity to the polypeptide of SEQ ID NO: 95; and
a glycoside hydrolase family 62 (GH62) polypeptides having arabinofuranosidase activity and at least 80% sequence identity to the polypeptide of SEQ ID NO: 27, wherein:
(a) the GH10 polypeptide and the GH62 polypeptide together solubilise at least 2.0% xylose from defatted destarched maize (DFDSM); and
(b) the GH10 polypeptide and the GH62 polypeptide together solubilise at least 2 times more xylose from DFDSM than the GH10 polypeptide can when the GH62 polypeptide is not present;
wherein (a) and (b) are performed under the reaction conditions:
i) 25 mg GH10 polypeptide per kg DFDSM,
ii) 12.5 mg GH62 polypeptide per kg DFDSM, and
iii) incubation at 40° C., pH 5 for 4 hours.
|