US 11,926,852 B2
Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
Wei Peng, Beijing (CN); Ninfa Rangel Pedersen, Soeborg (DK); Dan Pettersson, Lynge (DK); Jens Magnus Eklof, Copenhagen (DK); Soren Nymand-Grarup, Aabyhoej (DK); Lorena G. Palmen, Malmo (SE); Rune Nygaard Monrad, Hillerod (DK); Nikolaj Spodsberg, Bagsvaerd (DK); Mary Ann Stringer, Soeborg (DK); Charlotte Blom, Lynge (DK); Lars Kiemer, Ballerup (DK); Kristian Bertel Romer M. Krogh, Bagsvaerd (DK); and Jesper Salomon, Holte (DK)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Filed by Novozymes A/S, Bagsvaerd (DK)
Filed on May 28, 2021, as Appl. No. 17/333,898.
Application 16/887,004 is a division of application No. 15/528,910, granted, now 10,711,259, issued on Jul. 14, 2020, previously published as PCT/CN2015/097895, filed on Dec. 18, 2015.
Application 17/333,898 is a continuation of application No. 16/887,004, filed on May 29, 2020, granted, now 11,053,490.
Claims priority of application No. PCT/CN2014/094381 (WO), filed on Dec. 19, 2014; and application No. PCT/CN2015/071015 (WO), filed on Jan. 19, 2015.
Prior Publication US 2021/0332340 A1, Oct. 28, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/24 (2006.01); A23K 10/14 (2016.01); C12P 19/14 (2006.01)
CPC C12N 9/248 (2013.01) [A23K 10/14 (2016.05); C12N 9/24 (2013.01); C12N 9/2402 (2013.01); C12P 19/14 (2013.01); C12Y 302/01008 (2013.01); C12Y 302/01055 (2013.01); C07K 2319/02 (2013.01); C07K 2319/21 (2013.01); C07K 2319/55 (2013.01)] 18 Claims
 
1. A composition comprising:
a glycoside hydrolase family 10 (GH10) polypeptides having xylanase activity and at least 80% sequence identity to the polypeptide of SEQ ID NO: 95; and
a glycoside hydrolase family 62 (GH62) polypeptides having arabinofuranosidase activity and at least 80% sequence identity to the polypeptide of SEQ ID NO: 27, wherein:
(a) the GH10 polypeptide and the GH62 polypeptide together solubilise at least 2.0% xylose from defatted destarched maize (DFDSM); and
(b) the GH10 polypeptide and the GH62 polypeptide together solubilise at least 2 times more xylose from DFDSM than the GH10 polypeptide can when the GH62 polypeptide is not present;
wherein (a) and (b) are performed under the reaction conditions:
i) 25 mg GH10 polypeptide per kg DFDSM,
ii) 12.5 mg GH62 polypeptide per kg DFDSM, and
iii) incubation at 40° C., pH 5 for 4 hours.