	US 11,926,847 B2
	Selective enrichment of high-quality DNA intact sperms from a semen sample
Raunak Jay Soman, Hyderabad (IN); Jay Soman, Hyderabad (IN); and Motukuri Mahesh, Hyderabad (IN)
Assigned to Sanzyme Biologics Private Limited, Hyderabad (IN)
Appl. No. 17/045,471
Filed by Sanzyme Biologics Private Limited, Hyderabad (IN)
PCT Filed Apr. 3, 2019, PCT No. PCT/IN2019/050274 § 371(c)(1), (2) Date Oct. 5, 2020, PCT Pub. No. WO2019/193603, PCT Pub. Date Oct. 10, 2019.
Claims priority of application No. 201841004521 (IN), filed on Apr. 5, 2018.
Prior Publication US 2021/0155898 A1, May 27, 2021
Int. Cl. C12N 5/076 (2010.01); C12N 5/071 (2010.01)

CPC C12N 5/061 (2013.01) [C12N 5/0612 (2013.01); C12N 2500/05 (2013.01); C12N 2500/14 (2013.01); C12N 2500/16 (2013.01); C12N 2500/30 (2013.01); C12N 2500/32 (2013.01); C12N 2500/50 (2013.01); C12N 2500/84 (2013.01)]

4 Claims

1. A swim-up medium for selective enrichment of fractions containing DNA-intact sperm cells post swim-up having a DNA fragmentation index (DFI) with DFI_{post swim-up}<DFI_neat, from a semen sample, the swim-up medium comprising a solvent and a solute composition, said solute composition at a concentration of up to 15% w/v, and said solute composition further comprising:

a. a combination of electrolytes at a concentration range of 10% to 50% w/w consisting of sodium chloride, potassium chloride, magnesium sulphate, potassium di-hydrogen orthophosphate, calcium chloride, sodium bicarbonate, sodium phosphate monobasic anhydrous, magnesium chloride and glucose;

b. a combination of excipients at a concentration range of 1% to 30% w/w consisting of phenol red, HEPES buffer, gentamicin, sodium pyruvate, sodium lactate, glutamine, glycine, L-Arginine, and methionine;

c. Polyvinylpyrrolidone (PVP) at a concentration range of 3% to 80% w/w; and

d. recombinant Human Serum Albumin (rHSA) or bovine serum albumin (BSA) at a concentration range of 0.1% to 50% w/w;

wherein the said solvent is water.