	US 11,926,823 B2
	Compositions and methods of modifying a plant genome to produce a MS9, MS22, MS26, or MS45 male-sterile plant
Andrew Mark Cigan, Madison, WI (US); Tim Fox, Des Moines, IA (US); and Manjit Singh, Johnston, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., , IA (US)
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed on Nov. 11, 2021, as Appl. No. 17/454,510.
Application 17/454,510 is a continuation of application No. 16/214,897, filed on Dec. 10, 2018, granted, now 11,203,752.
Claims priority of provisional application 62/596,998, filed on Dec. 11, 2017.
Prior Publication US 2022/0056439 A1, Feb. 24, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/11 (2006.01); C07K 14/415 (2006.01); C12N 9/22 (2006.01); C12N 15/82 (2006.01)

CPC C12N 15/11 (2013.01) [C07K 14/415 (2013.01); C12N 9/22 (2013.01); C12N 15/8213 (2013.01); C12N 15/8289 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)]

13 Claims

1. A method for producing a male-sterile wheat plant, the method comprising:

a) introducing a genetic modification into at least one or more endogenous wheat MS9, MS22, MS26 or MS45 polynucleotide sequences in a wheat plant cell, wherein the genetic modification confers male sterility to a wheat plant from the plant cell, wherein the genetic modification is introduced by a Cas9 endonuclease guided by at least one guide RNA; and

b) obtaining the male-sterile wheat plant from the wheat plant cell.