US 11,926,820 B2
Methods and compositions for protein and peptide sequencing
Annalisa Marie Pawlosky, Mountain View, CA (US); Michael Gibbons, Mountain View, CA (US); Shirley Jing Shao, Sunnyvale, CA (US); Marc Berndl, Mountain View, CA (US); Michelle Therese Hoerner Dimon, Redwood City, CA (US); Ali Bashir, San Francisco, CA (US); and Lauren Schiff, Encino, CA (US)
Assigned to Google LLC, Mountain View, CA (US)
Filed by Google LLC, Mountain View, CA (US)
Filed on Sep. 11, 2020, as Appl. No. 17/019,084.
Claims priority of provisional application 62/900,447, filed on Sep. 13, 2019.
Prior Publication US 2021/0171937 A1, Jun. 10, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01)
CPC C12N 15/1048 (2013.01) 10 Claims
 
1. A method of identifying one or more binding events between a plurality of binders and a plurality of binding targets, comprising:
(a) incubating a plurality of known binding targets with a library of binders, where one or more binders within the library bind specifically to one or more binding targets, wherein each binder within the library comprises a target binder sequence and a unique barcode sequence indicative of the associated binder;
(b) ligating the unique barcode sequence that is specifically bound to a binding target onto a DNA barcode sequence proximal to the binding target, thereby generating a DNA barcode construct;
(c) removing the target binder sequence, thereby leaving the barcode sequence attached to the DNA barcode construct, such that the identity of the binder and, therefore, the identity of the binding target is identified;
(d) optionally, repeating steps (b)-(c);
(e) ligating sequencing adaptors onto the DNA barcode construct; and
(f) sequencing the DNA barcode construct,
thereby identifying one or more binding events between a plurality of binders and a plurality of binding targets.