US 11,925,726 B2
Perfusable-type dual proximal tubule cell construct and producing method thereof for applying in vitro artificialrenal tissue model and renal cell therapy
Dong-Woo Cho, Seoul (KR); Wonil Han, Pohang-si (KR); Narendra K. Singh, Pohang-si (KR); Yong Kyun Kim, Seongnam-si (KR); and Sun Ah Nam, Gimpo-si (KR)
Assigned to POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION, Pohang-si (KR); and THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY—ACADEMIC COOPERATION FOUNDATION, Seoul (KR)
Filed by POSTECH Research and Business Development Foundation, Pohang-si (KR); and THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Seoul (KR)
Filed on Dec. 18, 2020, as Appl. No. 17/126,449.
Claims priority of application No. 10-2020-0113999 (KR), filed on Sep. 7, 2020.
Prior Publication US 2022/0072203 A1, Mar. 10, 2022
Int. Cl. A61L 27/38 (2006.01); A61F 2/02 (2006.01); A61L 27/36 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01)
CPC A61L 27/3839 (2013.01) [A61F 2/022 (2013.01); A61L 27/3641 (2013.01); A61L 27/3683 (2013.01); A61L 27/3886 (2013.01); C12N 5/0685 (2013.01); C12N 5/0686 (2013.01); C12N 5/069 (2013.01); C12N 5/0697 (2013.01)] 20 Claims
 
1. A perfusable-type bio-dual proximal tubule cell construct comprising:
a first bioink comprising a decellularized substance derived from a mammalian kidney tissue and human umbilical vascular endothelial cells (HUVECs); and
a second bioink comprising the decellularized substance and renal proximal tubular epithelial cells (RPTECs),
wherein the first bioink and the second bioink are coaxial and printed in tubular constructs having different inner diameters.