CPC G01N 33/571 (2013.01) [G01N 33/543 (2013.01); G01N 33/54313 (2013.01); G01N 33/92 (2013.01); G01N 2333/20 (2013.01); G01N 2405/04 (2013.01); G01N 2469/20 (2013.01)] | 15 Claims |
1. A method of detecting the presence of syphilis antibodies in a sample comprising:
(a) contacting the sample with (i) a cardiolipin-lecithin-cholesterol antigen non-covalently attached to a solid support, wherein the cardiolipin-lecithin-cholesterol antigen is unmodified to reduce hydrophobicity; and (ii) a Treponema pallidum antigen attached to a solid support;
(b) washing the solid supports of (i) and (ii) to remove unbound sample;
(c) contacting the solid supports of (i) and (ii) with a labeled, anti-human immunoglobulin (Ig) antibody; and
(d) detecting the presence of syphilis antibodies by detecting the label on the anti- human Ig antibody.
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