US 11,913,138 B2
Generating recombinant affinity reagents with arrayed targets
Joshua Labaer, Chandler, AZ (US); Kevin Gorman, Chicago, IL (US); Brian Kay, Chicago, IL (US); Jie Wang, Tempe, AZ (US); and Ji Qiu, Chandler, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US); and THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, Urbana, IL (US)
Filed by ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US); and THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, Urbana, IL (US)
Filed on Aug. 18, 2021, as Appl. No. 17/405,172.
Application 17/405,172 is a continuation of application No. 15/748,946, granted, now 11,124,791, previously published as PCT/US2016/051514, filed on Sep. 13, 2016.
Claims priority of provisional application 62/218,362, filed on Sep. 14, 2015.
Prior Publication US 2021/0380970 A1, Dec. 9, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C40B 30/04 (2006.01); C12N 15/10 (2006.01); C12Q 1/6811 (2018.01)
CPC C40B 30/04 (2013.01) [C12N 15/1037 (2013.01); C12N 15/1041 (2013.01); C12N 15/1062 (2013.01); C12Q 1/6811 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method for affinity selection with arrayed target material, comprising the steps of:
a) providing an array comprising arrayed target proteins, wherein the arrayed target proteins comprise MAP2K5, CTBP1, SARA1A, CDK2 and RPS6KA3;
b) incubating a ribosome display material with the array,
c) washing the array to remove non-binding ribosome display material from the arrayed target proteins,
d) eluting from the arrayed target material any binding ribosome display material,
e) amplifying the binding ribosome display material; and
f) performing at least a further round of affinity selection repeating steps a)-d), whereby performing at least two rounds of affinity selection according to steps a) to d) yields bound ribosome display material having the highest affinity for at least one of the arrayed target proteins; and
g) isolating pools of the bound ribosome display material to identify ribosome display material having the highest affinity for the at least one of the arrayed target proteins.