US 11,913,064 B2
Molecular beacon-based optical gene biosensor employing retro-reflection and quantitative analysis method of nucleic acid molecule
Hyun-Chul Yoon, Seoul (KR); Jae-Ho Kim, Suwon-si (KR); Yong-Duk Han, Seongnam-si (KR); and Hyeong-Jin Chun, Suwon-si (KR)
Assigned to AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Suwon-si (KR)
Appl. No. 16/498,877
Filed by AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Suwon-si (KR)
PCT Filed Mar. 9, 2018, PCT No. PCT/KR2018/002812
§ 371(c)(1), (2) Date Sep. 27, 2019,
PCT Pub. No. WO2018/199465, PCT Pub. Date Nov. 1, 2018.
Claims priority of application No. 10-2017-0053397 (KR), filed on Apr. 26, 2017.
Prior Publication US 2021/0189471 A1, Jun. 24, 2021
Int. Cl. G01N 21/55 (2014.01); G02B 5/126 (2006.01); G02B 5/128 (2006.01); G02B 5/12 (2006.01); C12Q 1/6825 (2018.01)
CPC C12Q 1/6825 (2013.01) [G01N 21/55 (2013.01); G02B 5/126 (2013.01); G02B 5/128 (2013.01); G01N 2021/551 (2013.01); G01N 2201/0636 (2013.01); G02B 5/12 (2013.01)] 17 Claims
OG exemplary drawing
 
1. An optical gene biosensor comprising:
a substrate;
a molecular beacon anchored to the substrate, wherein the molecular beacon includes an oligonucleotide and a first compound, wherein the oligonucleotide is capable of specifically binding to a target nucleic acid, and wherein the first compound is bound to a first terminal of the oligonucleotide;
an optical marker configured to retro-reflect irradiated light;
a light source for irradiating the optical marker with light; and
a light-receiver for receiving light retro-reflected from the optical marker;
wherein the optical marker includes:
a second compound specifically binding to the first compound, wherein the second compound is coupled to a to-be-modified layer;
a transparent core particle;
a total-reflection inducing layer to cover a portion of a surface of the core particle, wherein the total-reflection inducing layer is made of a material having a refractive index lower than a refractive index of the core particle in a visible light wavelength range of 360 nm to 820 nm;
the to-be-modified layer formed on the total-reflection inducing layer; and
a magnetic layer made of magnetic material, wherein the magnetic layer is placed between the total-reflection inducing layer and the to-be-modified layer.