	US 11,913,061 B2
	Method for preparing nucleic acid sample
Takayoshi Inoue, Utsunomiya (JP); and Akira Hachiya, Utsunomiya (JP)
Assigned to KAO Corporation, Tokyo (JP)
Appl. No. 15/781,644
Filed by KAO CORPORATION, Tokyo (JP)
PCT Filed Jun. 7, 2017, PCT No. PCT/JP2017/021040 § 371(c)(1), (2) Date Jun. 5, 2018, PCT Pub. No. WO2018/008319, PCT Pub. Date Jan. 11, 2018.
Claims priority of application No. PCT/JP2016/070343 (WO), filed on Jul. 8, 2016.
Prior Publication US 2018/0371524 A1, Dec. 27, 2018
Int. Cl. C12Q 1/68 (2018.01); C07H 21/04 (2006.01); C12Q 1/6806 (2018.01); C12N 15/09 (2006.01); B32B 5/18 (2006.01); C12Q 1/6886 (2018.01); C12Q 1/6883 (2018.01); C12Q 1/686 (2018.01)

CPC C12Q 1/6806 (2013.01) [B32B 5/18 (2013.01); C12N 15/09 (2013.01); C12Q 1/68 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6883 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01); C12Q 2600/178 (2013.01)]

9 Claims

1. A method for preparing a nucleic acid derived from a human subject, comprising collecting a skin surface lipids sample comprising secretions secreted from a sebaceous gland of the human subject on a skin surface of the human subject and isolating the nucleic acid from the collected skin surface lipids sample, wherein the nucleic acid is human RNA and wherein said collecting comprises applying to the skin surface of the human subject a surface skin lipid absorbent material which is an oil-blotting film made of polypropylene, wherein the surface skin lipid absorbent material is not a surface skin lipid adhesive material.