US 11,913,054 B2
Fermentative production of n-butylacrylate using alcohol acyl transferase enzymes
Stephan Saum, Lampertheim (DE); Woncheol Kim, Tarrytown, NY (US); Oskar Zelder, Ludwigshafen (DE); Jennifer Jaitzig, Ludwigshafen (DE); and Zheyuan Guo, San Diego, CA (US)
Assigned to BASF SE, Ludwigshafen am Rhein (DE)
Appl. No. 16/089,642
Filed by BASF SE, Ludwigshafen am Rhein (DE)
PCT Filed Mar. 22, 2017, PCT No. PCT/EP2017/056872
§ 371(c)(1), (2) Date Sep. 28, 2018,
PCT Pub. No. WO2017/167623, PCT Pub. Date Oct. 5, 2017.
Claims priority of application No. 16162887 (EP), filed on Mar. 30, 2016.
Prior Publication US 2019/0112622 A1, Apr. 18, 2019
Int. Cl. C12P 7/62 (2022.01); C12N 9/10 (2006.01)
CPC C12P 7/62 (2013.01) [C12N 9/1029 (2013.01); C12Y 203/01084 (2013.01)] 4 Claims
 
1. A method for fermentative production of n-butylacrylate (n-BA) comprising the steps of:
i) providing a recombinant microorganism comprising a butanol producing pathway and an acryloyl-CoA producing pathway and expressing an alcohol acyl transferase (AAT) gene encoding an AAT enzyme having an n-BA forming activity;
ii) culturing said microorganism under conditions that allow for the production of n-BA; and
iii) recovering n-BA from a fermentation broth;
wherein the AAT gene encoding an AAT enzyme having an n-BA forming activity is selected from the group consisting of:
(I) a nucleic acid molecule comprising a sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11 and 13;
(II) a nucleic acid molecule having at least 95% identity to a nucleic acid molecule selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11 and 13;
(III) a nucleic acid molecule hybridizing to the full complement of a nucleic acid molecule having a sequence selected from the group consisting of SEQ ID NO: 1, 5, 7, 9, 11 and 13;
(IV) a nucleic acid molecule encoding a polypeptide selected from the group consisting of SEQ ID NO: 2, 6, 8, 10, 12, and 14; and
(V) a nucleic acid molecule encoding a polypeptide having at least 95% identity to a polypeptide selected from the group consisting of SEQ ID NO: 2, 6, 8, 10, 12, and 14.