CPC C12P 21/02 (2013.01) [C12N 15/67 (2013.01)] | 15 Claims |
1. A cell-free method for synthesizing a non-ribosomal peptide target protein without the use of a transcription or translation template encoding the target protein, the method comprising:
a) contacting a translation template encoding a non-ribosomal peptide synthase (“NRPS”) that is specific for the synthesis of the target protein with a cellular extract from a host strain, the extract comprising natural enzyme metabolism from the host strain;
wherein the translation template is translated once it is contacted with the cellular extract, thereby providing a NRPS-containing extract;
b) contacting the NRPS-containing extract to a feedstock, the feedstock comprising components for the formation of the target protein.
|