US 11,912,989 B2
Antigen-binding molecules, the antigen-binding activity of which varies according to the concentration of compounds, and libraries of said molecules
Tomoyuki Igawa, Shizuoka (JP); Shigero Tamba, Shizuoka (JP); Shun Shimizu, Shizuoka (JP); Kanako Tatsumi, Shizuoka (JP); Shojiro Kadono, Kanagawa (JP); Hiroki Kawauchi, Kanagawa (JP); Kazuhiro Ohara, Kanagawa (JP); Masayuki Matsushita, Kanagawa (JP); Takashi Emura, Shizuoka (JP); and Masaki Kamimura, Shizuoka (JP)
Assigned to Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
Filed by CHUGAI SEIYAKU KABUSHIKI KAISHA, Tokyo (JP)
Filed on Feb. 23, 2021, as Appl. No. 17/182,331.
Application 17/182,331 is a division of application No. 15/100,934, granted, now 10,961,530, previously published as PCT/JP2014/082060, filed on Dec. 4, 2014.
Claims priority of application No. JP2013-251537 (JP), filed on Dec. 4, 2013.
Prior Publication US 2021/0180049 A1, Jun. 17, 2021
Int. Cl. C40B 40/10 (2006.01); C12N 15/10 (2006.01); C07K 16/28 (2006.01); C07K 16/24 (2006.01); C07K 16/00 (2006.01); C07K 16/42 (2006.01); C07K 16/44 (2006.01); C40B 50/06 (2006.01); C07K 16/30 (2006.01)
CPC C12N 15/1093 (2013.01) [C07K 16/00 (2013.01); C07K 16/005 (2013.01); C07K 16/248 (2013.01); C07K 16/2809 (2013.01); C07K 16/2863 (2013.01); C07K 16/2866 (2013.01); C07K 16/30 (2013.01); C07K 16/4283 (2013.01); C07K 16/44 (2013.01); C40B 50/06 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/55 (2013.01); C07K 2317/92 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A library that comprises:
(i) a plurality of antigen-binding domains having different amino acid sequences from one another, or
(ii) a plurality of nucleic acids that encode a plurality of antigen-binding domains having different amino acid sequences from one another, or
(iii) a plurality of antigen-binding molecules each comprising an antigen-binding domain, which have different amino acid sequences from one another, or
(iv) a plurality of nucleic acids that encode a plurality of antigen-binding molecules each comprising an antigen-binding domain, which have different amino acid sequences from one another,
wherein the plurality of antigen-binding domains or antigen-binding domains contained in the plurality of antigen-binding molecules comprises a first antigen-binding domain whose binding affinity for a first antigen varies depending on the concentration of a small molecule compound; and wherein the first antigen is not the small molecule compound.