CPC C12N 15/1086 (2013.01) [C07K 14/70596 (2013.01); C07K 14/7153 (2013.01); C07K 16/2863 (2013.01); C07K 16/2896 (2013.01); C12N 5/0636 (2013.01); C12N 5/0693 (2013.01); C12N 9/22 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/53 (2013.01); C12N 2501/599 (2013.01); C12N 2502/1114 (2013.01); C12N 2502/30 (2013.01); C40B 30/04 (2013.01)] | 15 Claims |
1. A method comprising:
(a) co-culturing (i) engineered tumor cells that comprise an extracellular protein and (ii) edited T cells that comprise a chimeric antigen receptor (CAR) that comprises an extracellular target-binding domain that binds to the extracellular protein, wherein the edited T cells comprise a Cas9 protein and a guide RNA (gRNA) library that comprises gRNAs targeting one or more genomic loci in the edited T cells; and
(b) isolating the edited T cells that accumulate during the co-culturing.
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