	US 11,912,980 B2
	Method for the production of a dairy food product and a method for gene transfer by conjugation
Mariya Alexeevna Tarazanova, Zwolle (NL); Thom Huppertz, Ede (NL); Herwig Bachmann, Amsterdam (NL); Jan Kok, Groningen (NL); and Marke Beerthuyzen, Ede (NL)
Assigned to DSM IP Assets B.V., Heerlen (NL)
Filed by DSM IP ASSETS B.V., Heerlen (NL)
Filed on Jun. 18, 2021, as Appl. No. 17/351,548.
Application 17/351,548 is a division of application No. 16/084,591, filed on Sep. 13, 2018, granted, now 11,066,639.
Prior Publication US 2021/0324324 A1, Oct. 21, 2021
Int. Cl. C12N 1/20 (2006.01); C07K 14/315 (2006.01); A23L 33/135 (2016.01); A23C 9/123 (2006.01); C07K 14/195 (2006.01); C12R 1/46 (2006.01)

CPC C12N 1/20 (2013.01) [A23C 9/1236 (2013.01); A23L 33/135 (2016.08); C07K 14/195 (2013.01); C07K 14/315 (2013.01); C12N 1/205 (2021.05); A23V 2400/131 (2023.08); A23V 2400/157 (2023.08); A23V 2400/249 (2023.08); C12R 2001/46 (2021.05)]

12 Claims

1. A method for gene transfer by conjugation comprising

a) providing a composition comprising at least two bacterial strains, wherein at least one bacterial strain comprises an increased amount of expression product of a pilin gene cluster compared to that of Lactococcus lactis subsp. cremoris NCDO 712 when cultured under identical conditions, and

b) incubating the composition under conditions conducive to conjugation.