CPC C12M 41/48 (2013.01) [C12M 23/28 (2013.01); C12M 23/44 (2013.01); C12M 27/00 (2013.01); C12M 29/00 (2013.01); C12M 29/04 (2013.01); C12M 35/00 (2013.01); C12M 37/02 (2013.01); C12M 41/12 (2013.01); C12M 41/36 (2013.01); C12M 45/02 (2013.01); C12M 47/04 (2013.01); C12N 5/0634 (2013.01); C12N 5/0696 (2013.01)] | 30 Claims |
1. A method for producing an induced pluripotent stem cell comprising:
transporting a cell-containing solution comprising cells through a preintroduction cell solution-feeding channel to a factor introducing device connected to the preintroduction cell solution-feeding channel,
transporting pluripotency inducing factors from a factor storing unit that preserves the pluripotency inducing factors to the factor introducing device through a factor solution-feeding channel,
introducing the pluripotency inducing factors into the cells to prepare cells comprising factor-introduced cells in the factor introducing device, and
culturing the factor-introduced cells to prepare a cell mass or a plurality of cell masses comprising induced pluripotent stem cells in a cell mass preparation device,
wherein the cell mass preparation device comprises an initializing culturing apparatus that cultures the factor-introduced cells, and
wherein the factor introducing device is non-rotatable.
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