US 11,912,977 B2
Pluripotent stem cell production system
Koji Tanabe, Palo Alto, CA (US); Brendan Kelly, Palo Alto, CA (US); Kenta Suto, Palo Alto, CA (US); Hidenori Shimoda, Palo Alto, CA (US); and Ryoji Hiraide, Palo Alto, CA (US)
Assigned to I Peace, Inc., Palo Alto, CA (US)
Filed by I Peace, Inc., Palo Alto, CA (US); and Koji Tanabe, Palo Alto, CA (US)
Filed on Aug. 3, 2022, as Appl. No. 17/817,287.
Application 17/817,287 is a continuation of application No. 15/756,031, granted, now 11,518,974, previously published as PCT/JP2016/075540, filed on Aug. 31, 2016.
Claims priority of provisional application 62/356,199, filed on Jun. 29, 2016.
Claims priority of application No. 2015-170797 (JP), filed on Aug. 31, 2015.
Prior Publication US 2022/0389368 A1, Dec. 8, 2022
Int. Cl. C12N 5/074 (2010.01); C12M 1/36 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 1/33 (2006.01); C12M 1/42 (2006.01); C12N 5/078 (2010.01); C12M 3/00 (2006.01); C12M 1/02 (2006.01); C12M 1/12 (2006.01)
CPC C12M 41/48 (2013.01) [C12M 23/28 (2013.01); C12M 23/44 (2013.01); C12M 27/00 (2013.01); C12M 29/00 (2013.01); C12M 29/04 (2013.01); C12M 35/00 (2013.01); C12M 37/02 (2013.01); C12M 41/12 (2013.01); C12M 41/36 (2013.01); C12M 45/02 (2013.01); C12M 47/04 (2013.01); C12N 5/0634 (2013.01); C12N 5/0696 (2013.01)] 30 Claims
OG exemplary drawing
 
1. A method for producing an induced pluripotent stem cell comprising:
transporting a cell-containing solution comprising cells through a preintroduction cell solution-feeding channel to a factor introducing device connected to the preintroduction cell solution-feeding channel,
transporting pluripotency inducing factors from a factor storing unit that preserves the pluripotency inducing factors to the factor introducing device through a factor solution-feeding channel,
introducing the pluripotency inducing factors into the cells to prepare cells comprising factor-introduced cells in the factor introducing device, and
culturing the factor-introduced cells to prepare a cell mass or a plurality of cell masses comprising induced pluripotent stem cells in a cell mass preparation device,
wherein the cell mass preparation device comprises an initializing culturing apparatus that cultures the factor-introduced cells, and
wherein the factor introducing device is non-rotatable.