	US 11,912,924 B2
	Composition for antifreezing
Dong June Ahn, Seoul (KR); Sang Yup Lee, Seoul (KR); and Yong Ho Cho, Seoul (KR)
Assigned to Korea University Research and Business Foundation, Seoul (KR)
Filed by Korea University Research and Business Foundation, Seoul (KR)
Filed on Dec. 3, 2019, as Appl. No. 16/702,245.
Claims priority of application No. 10-2019-0154268 (KR), filed on Nov. 27, 2019.
Prior Publication US 2021/0155495 A1, May 27, 2021
Int. Cl. A01N 1/02 (2006.01); C09K 5/20 (2006.01); A23L 3/37 (2006.01); C01G 7/00 (2006.01); C09K 3/18 (2006.01); B22F 1/054 (2022.01)

CPC C09K 5/20 (2013.01) [A01N 1/0221 (2013.01); A01N 1/0284 (2013.01); A23L 3/37 (2013.01); C01G 7/00 (2013.01); C09K 3/18 (2013.01); B22F 1/0553 (2022.01); C01P 2004/12 (2013.01); C01P 2004/41 (2013.01); C01P 2004/64 (2013.01)]

17 Claims

1. A method for freezing a cell or tissue, the method comprising exposing a cell or tissue of a subject to a temperature below zero in the presence of a cryopreservation composition comprising a cryoprotective agent consisting of a gold (Au) nano structure in which at least a portion thereof is concave, wherein the gold (Au) nanostructure has a concave surface such that a liquid water layer is formed thereon and ice does not grow on the concave surface during the exposing the cell or tissue of the subject to the temperature below zero in the presence of the cryopreservation composition, and wherein the gold (Au) nanostructure has a shape of tetrahedron, hexahedron, octahedron, decahedron, dodecahedron, icosahedron, tetrakis hexahedron, hexakis octahedron, rhombic dodecahedron, cylinder, saddle or cap.