US 11,911,452 B2
Multivalent pneumococcal polysaccharide-protein conjugate composition
Kyungjun An, Seoul (KR); Dongsoo Ham, Suwon-si (KR); Hun Kim, Suwon-si (KR); Sunghyun Kim, Yongin-si (KR); Jinhwan Shin, Seoul (KR); Robert Hopfer, Roaring Brook Township, PA (US); Richard D. Kensinger, Henryville, PA (US); Moe Kyaw, Swiftwater, PA (US); and Philippe Talaga, Lyons (FR)
Assigned to SANOFI PASTEUR INC., Swiftwater, PA (US); and SK BIOSCIENCE CO., LTD., Seoul (KR)
Filed by SANOFI PASTEUR INC., Swiftwater, PA (US); and SK BIOSCIENCE CO., LTD., Seongnam-si (KR)
Filed on Sep. 10, 2021, as Appl. No. 17/471,825.
Application 17/471,825 is a continuation of application No. 16/966,584, granted, now 11,123,417, previously published as PCT/US2019/016511, filed on Feb. 4, 2019.
Claims priority of provisional application 62/626,509, filed on Feb. 5, 2018.
Claims priority of application No. 10-2018-0045246 (KR), filed on Apr. 18, 2018.
Prior Publication US 2021/0401963 A1, Dec. 30, 2021
Int. Cl. C12P 21/06 (2006.01); A61K 39/09 (2006.01); A61K 9/00 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/092 (2013.01) [A61K 9/0019 (2013.01); A61K 2039/6031 (2013.01); A61K 2039/70 (2013.01)] 9 Claims
 
1. A method for preparing an immunogenic serotype 9N conjugate, the method comprising:
(a) lysing a bacterial cell producing Streptococcus pneumoniae serotype 9N capsular polysaccharide by fermentation;
(b) purifying Streptococcus pneumoniae serotype 9N capsular polysaccharide from the lysed bacterial cell;
(c) activating the Streptococcus pneumoniae serotype 9N capsular polysaccharide by reacting with an oxidizing agent to achieve a degree of oxidation of 2-19, wherein the activated Streptococcus pneumoniae serotype 9N capsular polysaccharide has a molecular weight of 200-700 kDa; and
(d) mixing the activated Streptococcus pneumoniae serotype 9N capsular polysaccharide with a CRM197 carrier protein to conjugate the CRM197 carrier protein to the activated Streptococcus pneumoniae serotype 9N capsular polysaccharide and thereby form the immunogenic serotype 9N conjugate.