CPC A61K 39/092 (2013.01) [A61K 9/0019 (2013.01); A61K 2039/6031 (2013.01); A61K 2039/70 (2013.01)] | 9 Claims |
1. A method for preparing an immunogenic serotype 9N conjugate, the method comprising:
(a) lysing a bacterial cell producing Streptococcus pneumoniae serotype 9N capsular polysaccharide by fermentation;
(b) purifying Streptococcus pneumoniae serotype 9N capsular polysaccharide from the lysed bacterial cell;
(c) activating the Streptococcus pneumoniae serotype 9N capsular polysaccharide by reacting with an oxidizing agent to achieve a degree of oxidation of 2-19, wherein the activated Streptococcus pneumoniae serotype 9N capsular polysaccharide has a molecular weight of 200-700 kDa; and
(d) mixing the activated Streptococcus pneumoniae serotype 9N capsular polysaccharide with a CRM197 carrier protein to conjugate the CRM197 carrier protein to the activated Streptococcus pneumoniae serotype 9N capsular polysaccharide and thereby form the immunogenic serotype 9N conjugate.
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