CPC G01N 21/3151 (2013.01) [A61B 5/0075 (2013.01); A61B 5/1451 (2013.01); A61B 5/1455 (2013.01); A61B 5/14532 (2013.01); G01N 21/3577 (2013.01); G01N 33/487 (2013.01); A61B 5/0062 (2013.01); A61B 2562/0238 (2013.01); G01N 21/21 (2013.01); G01N 33/49 (2013.01); G01N 2021/3148 (2013.01); G01N 2201/10 (2013.01)] | 20 Claims |
1. A process for quantifying a concentration of a targeted molecule in a liquid sample through use of an absorption spectroscopy process, comprising:
(1) pulsing a signal beam with a signal pulsed source;
(2) pulsing a reference beam with a reference pulsed source;
(3) spatially combining the pulsed signal beam and the pulsed reference beam into a single radiation beam which passes into the liquid sample to a first effective depth and then is reflected out of the liquid sample;
(4) detecting a pulsed signal beam output and a pulsed reference beam output after the single radiation beam passes out of the liquid sample;
(5) processing the pulsed signal beam output and the pulsed reference beam output to obtain a value over a preselected period of time;
(6) repeating steps (1) through (5) for at least one additional effective depth to obtain a sampling dataset; and
(7) calculating a concentration level of the targeted particle in the liquid sample based on the sampling dataset;
wherein the signal pulsed source emits radiation at the signal bandwidth which is coincident with an absorption band of the targeted particle while the reference pulsed source emits radiation at a reference wavelength which is not coincident with the absorption band; and
wherein the signal pulsed source is separate from the reference pulsed source.
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