	US 11,891,670 B2
	Probes, microarray, primers, kit and applications for rapid detection of clinical ophthalmic microorganisms
Yusen Huang, Qingdao (CN); Bining Zhang, Qingdao (CN); Wenfeng Li, Qingdao (CN); Qing Liu, Qingdao (CN); Zhichao Ren, Qingdao (CN); and Huabo Chen, Qingdao (CN)
Assigned to AFFILIATED EYE INSTITUTE OF SHANDONG FIRST MEDICAL UNIVERSITY, Qingdao (CN)
Filed by Affiliated Eye Institute of Shandong First Medical University, Qingdao (CN)
Filed on Sep. 6, 2022, as Appl. No. 17/929,875.
Application 17/929,875 is a continuation of application No. PCT/CN2021/137383, filed on Dec. 13, 2021.
Claims priority of application No. 202011604557.8 (CN), filed on Dec. 30, 2020.
Prior Publication US 2023/0008908 A1, Jan. 12, 2023
Int. Cl. C12Q 1/689 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6844 (2018.01); C07H 21/00 (2006.01)

CPC C12Q 1/689 (2013.01) [C12Q 1/6816 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6844 (2013.01)]

2 Claims

1. A primer-probe set for rapid detection of clinical ophthalmic microorganisms, comprising probes and primers;

wherein the probes comprise probes for detecting Bacillus subtilis;

the probes for detecting Bacillus subtilis comprise probes with nucleotide sequences as shown in SEQ ID NO.141-147 respectively;

the primers comprise primers for detecting Bacillus subtilis;

the primers for detecting Bacillus subtilis comprise a forward primer with a nucleotide sequence as shown in SEQ ID NO.171 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO.172;

wherein a 5′ end of every forward primer in the primers is labeled with fluorescent group, and the fluorescent group comprises Cyanine 3 (Cy3).