CPC C12Q 1/6883 (2013.01) [C12Q 2600/158 (2013.01); G01N 1/28 (2013.01); G01N 33/6893 (2013.01); G01N 2800/245 (2013.01); G01N 2800/347 (2013.01)] | 8 Claims |
1. A method of treating a kidney transplant rejection in a patient, the method comprising the steps of:
a) isolating a microvesicle fraction from a urine sample from the patient;
b) extracting nucleic acids from the microvesicle fraction;
c) determining the expression levels of at least two biomarkers from the extracted nucleic acids, wherein the at least two biomarkers comprise each of CXCL9 and CXCL10, and the expression level of at least one normalizing gene, wherein the at least one normalizing gene comprises IL17RA;
d) normalizing the expression levels of the at least two biomarkers to the expression level of the at least one normalizing gene;
e) determining that the patient is undergoing a kidney transplant rejection based on the normalized expression levels of the at least two biomarkers; and
f) administering at least one kidney transplant rejection therapy to the patient undergoing a kidney transplant rejection.
|