US 11,891,663 B2
Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
Caifu Chen, Palo Alto, CA (US); Dana Ridzon, San Ramon, CA (US); Zhaohui Zhou, San Ramon, CA (US); Kai Qin Lao, Pleasanton, CA (US); and Neil A. Straus, Emeryville, CA (US)
Assigned to APPLIED BIOSYSTEMS, LLC, Carlsbad, CA (US)
Filed by APPLIED BIOSYSTEMS, LLC, Carlsbad, CA (US)
Filed on Aug. 26, 2020, as Appl. No. 16/947,974.
Application 16/947,974 is a division of application No. 15/490,323, filed on Apr. 18, 2017, granted, now 10,781,486.
Application 15/490,323 is a division of application No. 15/009,681, filed on Jan. 28, 2016, granted, now 9,657,346, issued on May 23, 2017.
Application 15/009,681 is a continuation of application No. 13/612,485, filed on Sep. 12, 2012, abandoned.
Application 13/612,485 is a continuation of application No. 12/543,466, filed on Aug. 18, 2009, granted, now 9,068,222, issued on Jun. 30, 2015.
Application 12/543,466 is a continuation of application No. 10/947,460, filed on Sep. 21, 2004, granted, now 7,575,863, issued on Aug. 18, 2009.
Claims priority of provisional application 60/575,661, filed on May 28, 2004.
Prior Publication US 2020/0407789 A1, Dec. 31, 2020
Int. Cl. C12Q 1/6876 (2018.01); C12N 9/00 (2006.01); C07H 21/04 (2006.01); C12Q 1/686 (2018.01); C12P 19/34 (2006.01)
CPC C12Q 1/6876 (2013.01) [C07H 21/04 (2013.01); C12N 9/00 (2013.01); C12P 19/34 (2013.01); C12Q 1/686 (2013.01); C12Q 2525/301 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/178 (2013.01)] 8 Claims
 
1. A linker probe comprising a stem, a loop, and a 3′ target-specific portion, wherein the 3′ target-specific portion is capable of base pairing with the 3′ end region of an miRNA target nucleic acid, and wherein the 3′ end of the linker probe is extensible.