US 11,891,661 B2
Non-destructive bilayer monitoring using measurement of bilayer response to electrical stimulus
Kevin Aliado, Sunnyvale, CA (US); Roger J. A. Chen, Saratoga, CA (US); Jing Luo, Pleasanton, CA (US); William Nielsen, San Jose, CA (US); Kyle Umeda, Saratoga, CA (US); and Ashraf Wahba, Hayward, CA (US)
Assigned to Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed by Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed on Dec. 20, 2022, as Appl. No. 18/068,620.
Application 18/068,620 is a continuation of application No. 16/892,212, filed on Jun. 3, 2020, granted, now 11,530,443.
Application 16/892,212 is a continuation of application No. 16/186,894, filed on Nov. 12, 2018, granted, now 10,683,543, issued on Jun. 16, 2020.
Application 16/186,894 is a continuation of application No. 15/085,700, filed on Mar. 30, 2016, granted, now 10,155,979, issued on Dec. 18, 2018.
Prior Publication US 2023/0120047 A1, Apr. 20, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 27/327 (2006.01); C12Q 1/6869 (2018.01); G01N 33/487 (2006.01)
CPC C12Q 1/6869 (2013.01) [G01N 33/48721 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method of detecting whether a membrane has thinned in a cell of a nanopore based sequencing chip, comprising:
applying a first voltage across a membrane disposed between a working electrode and a counter electrode, the membrane electrically coupled with a capacitor;
periodically sampling a second voltage across the capacitor;
determining a change in the sampled second voltage across the capacitor in response to a change in the first voltage; and
detecting that the membrane has been thinned at least partially into a thin membrane based on the determined change in the sampled second voltage across the capacitor in response to the change in the first voltage, wherein the thin membrane has a thickness that allows a nanopore to insert into the thin membrane and form an electrical path between the working electrode and the counter electrode.