US 11,891,660 B2
Linked paired strand sequencing
Eli N. Glezer, Del Mar, CA (US); Martin Maria Fabani, Encinitas, CA (US); Sabrina Shore, San Diego, CA (US); and Daan Witters, San Diego, CA (US)
Assigned to Singular Genomics Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc., San Diego, CA (US)
Filed on Nov. 17, 2022, as Appl. No. 18/056,348.
Application 17/500,733 is a division of application No. 17/194,023, filed on Mar. 5, 2021, granted, now 11,359,238, issued on Jun. 14, 2022.
Application 18/056,348 is a continuation of application No. 17/500,733, filed on Oct. 13, 2021.
Claims priority of provisional application 63/087,125, filed on Oct. 2, 2020.
Claims priority of provisional application 63/020,881, filed on May 6, 2020.
Claims priority of provisional application 62/986,527, filed on Mar. 6, 2020.
Prior Publication US 2023/0203576 A1, Jun. 29, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01); C12Q 1/6855 (2018.01)
CPC C12Q 1/6869 (2013.01) [C12Q 1/6855 (2013.01)] 20 Claims
 
1. A method of forming a template polynucleotide on a substrate, said method comprising:
a) ligating a first end of a double-stranded nucleic acid to a first hairpin adapter comprising a first primer binding sequence, wherein said first adapter is attached to said substrate;
b) ligating a second adapter to a second end of the double-stranded nucleic acid, wherein the second adapter comprises a second primer binding sequence and a third primer binding sequence, thereby forming said template polynucleotide on said substrate;
wherein the first primer binding sequence, the second primer binding sequence, and the third primer binding sequence are different.