CPC C12Q 1/6869 (2013.01) [C12Q 1/6855 (2013.01)] | 20 Claims |
1. A method of forming a template polynucleotide on a substrate, said method comprising:
a) ligating a first end of a double-stranded nucleic acid to a first hairpin adapter comprising a first primer binding sequence, wherein said first adapter is attached to said substrate;
b) ligating a second adapter to a second end of the double-stranded nucleic acid, wherein the second adapter comprises a second primer binding sequence and a third primer binding sequence, thereby forming said template polynucleotide on said substrate;
wherein the first primer binding sequence, the second primer binding sequence, and the third primer binding sequence are different.
|