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1. A protease variant, comprising a substitution at one or more positions corresponding to positions 1, 5, 9, 25, 29, 30, 32, 38, 39, 40, 41, 42, 45, 46, 49, 50, 53, 54, 55, 57, 59, 60, 61, 62, 64, 67, 68, 69, 74, 76, 77, 78, 79, 80, 81, 82, 87, 93, 94, 96, 99, 102, 103, 105, 109, 111, 112, 114, 115, 117, 122, 123, 124, 128, 130, 136, 141, 142, 145, 146, 147, 150, 153, 154, 157, 159, 161, 167, 169, 175, 182, 185, 199, 200, 205, 207, 209, 210, 213, 216, 217, 225, 228, 231, 234, 237, 242, 244, 245, 246, 248, 258, 262, 266, 267, 269, 271, 272, 276, 278, 280, 284, 289, 293, 296, 299, 305, 308, 313, 314, 317, 318, 319, 321, 322, 324, 325, 326, 329, 330, 331, 333, 336, 338, 341, 343, 345, 347, 348, 355, 358, 359, 361, 362, 363, and 364 of the polypeptide of SEQ ID NO: 3, and the variant has protease activity and wherein the variant has at least 90%, but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 3, and wherein the variant has increased thermo-stability measured as improvement factor, HIF, compared to the protease of SEQ ID NO: 3, wherein the at least one substitution is selected from the group consisting of: A1T, S5I, S5K, S5L, S5Y, T9H, K25P, S29I, S29P, S29R, S30E, K32W, F38L, I39G, I39L, I39V, I39Y, D40F, D40G, D40H, D40N, D40P, Q41I, Q41V, F42C, F42K, F42S, F42Y, K45L, K45M, K45Y, A46C, A46E, K49P, S50C, S50I, S50L, S50M, S50Q, A53C, A53E, A53V, Q54I, Q54L, Q54M, Q54V, F55H, F55L, K57P, K57S, I59G, I59M, I59P, S60P, S61L, S61P, S62C, S62N, S62P, T64G, T64I, L67V, Q68V, Q68Y, T69C, E74H, E74R, D76L, D76R, Q77L, S78D, P79Y, S80L, E81A, E81H, E81K, E81L, E81P, E81Y, A82F, A82L, A82M, A82P, N87S, T93L, T93S, V94S, L96W, G99C, T102L, T102V, T103I, T103K, T103V, I105Q, D109H, D109K, D109M, D109Y, F111H, F111K, F111S, F111Y, Q112R, G114L, N115A, N115C, N115K, N115P, N115R, N115T, N115Y, E117D, E117G, E117H, E117N, E117Q, E117S, I122K, I122R, I123A, N124L, N124V, G128A, G128E, G128F, G128L, G128S, S130D, S130G, S130L, S130N, S130P, L136K, G141T, Q142A, Q142F, Q142L, Q142N, N145V, T146A, T146I, T146L, T146N, I147S, K150F, K150L, K150R, N153A, N153L, N153M, N153P, N153R, N153Y, Q154A, Q154I, Q154L, Q154M, Q154N, Q154T, Q154V, N157I, N157L, Y159H, Q161E, T167F, I169F, I169S, D175I, D175N, Q182C, Q182T, Q182V, H185P, F199L, F199M, M200S, A205S, Q207H, Q207L, V209F, 5210T, T213L, T213R, A216R, F217I, F217Y, V225Y, I228L, I228T, I228W, Y231I, S234Q, S237F, S237Q, A242I, A242L, A242V, G244S, S245P, T246S, S248Y, F258P, S262P, V266K, V266R, D267A, D267H, D267L, D267N, Q269L, V271R, S272I, S272K, S272L, S272R, S272T, S272V, S272Y, T276L, T276Y, G278P, D280N, D280S, D280T, D280Y, C284A, C284G, F289Y, I293T, V296A, V296I, R299N, K305S, L308V, P313L, F314I, F314L, S317E, S317N, S317W, S318P, A319F, A319Q, K321L, K321Q, K321S, A322D, L324Q, L324S, N325L, D326P, S329C, S329H, S329I, S329L, S329N, S329T, S329V, S329W, G330Q, S331W, S331Y, P333R, S336K, S336N, N338M, N338Q, N338R, P341S, K343A, K343H, K343S, K343T, K343V, G345S, D347I, P348Y, P355I, P355R, A358L, A358P, A358R, A358Y, K359L, K359S, L361M, T362L, T362N, A363G, A363L, and V364I, wherein the positions correspond to amino acid positions in the amino acid sequence set forth in SEQ ID NO: 3; and wherein the substitution at the one or more positions provides a protease variant having an increase in thermo-stability measured as improvement factor, HIF, of at least 1.4.
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