US 11,891,643 B2
Methods and systems for monomer chain formation
Michael Dubrovsky, Somerville, MA (US)
Assigned to SiPhox, Inc.
Filed by SiPhox, Inc., Burlington, MA (US)
Filed on Jun. 3, 2021, as Appl. No. 17/337,931.
Claims priority of provisional application 63/034,205, filed on Jun. 3, 2020.
Prior Publication US 2021/0381018 A1, Dec. 9, 2021
Int. Cl. C12P 19/34 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01)
CPC C12P 19/34 (2013.01) [C12N 9/22 (2013.01); C12N 15/111 (2013.01); C12N 2310/20 (2017.05)] 18 Claims
 
1. A method of monomer chain formation comprising:
immobilizing, using a surface of a waveguide, a monomer chain, wherein the surface of the waveguide further comprises a plurality of probes, wherein at least a probe of the plurality of probes comprises a high-contrast agent;
providing a first monomer, wherein the first monomer comprises a capping agent;
attaching the first monomer to the monomer chain; and
detecting, using a sensor in communication with the waveguide, presence of the first monomer, wherein detecting further comprises:
introducing a primary cleaving agent proximal the plurality of probes, wherein the primary cleaving agent is configured to selectively cleave the at least a probe as a result of the primary cleaving agent being in presence of an analyte; and
introducing a secondary cleaving agent configured to selectively cleave at least an additional probe as a result of the secondary cleaving agent being in the presence of the analyte.