CPC C12P 19/34 (2013.01) [C12N 9/22 (2013.01); C12N 15/111 (2013.01); C12N 2310/20 (2017.05)] | 18 Claims |
1. A method of monomer chain formation comprising:
immobilizing, using a surface of a waveguide, a monomer chain, wherein the surface of the waveguide further comprises a plurality of probes, wherein at least a probe of the plurality of probes comprises a high-contrast agent;
providing a first monomer, wherein the first monomer comprises a capping agent;
attaching the first monomer to the monomer chain; and
detecting, using a sensor in communication with the waveguide, presence of the first monomer, wherein detecting further comprises:
introducing a primary cleaving agent proximal the plurality of probes, wherein the primary cleaving agent is configured to selectively cleave the at least a probe as a result of the primary cleaving agent being in presence of an analyte; and
introducing a secondary cleaving agent configured to selectively cleave at least an additional probe as a result of the secondary cleaving agent being in the presence of the analyte.
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