US 11,891,642 B2
Biocatalytic techniques
Jonathan Charles Paul Steele, Maidenhead (GB); Antonio De Riso, London (GB); Francesco Falcioni, Manchester (GB); Richard Kerry Phipps, Aylesbury (GB); Stephen Keith Wrigley, High Wycombe (GB); Emily Jade Hopkins, Reading (GB); Aksana Rimu Khan, Slough (GB); Tetsuo Kokubun, Egham (GB); Kinga Linda Nytko, Slough (GB); Vincent Poon, Bicester (GB); Sebastian Schulz, Schenkendoebern (DE); John Maxim Ward, London (GB); and Mariacristina Bawn, Royston (GB)
Assigned to HYPHA DISCOVERY LIMITED, Abingdon (GB)
Appl. No. 17/296,772
Filed by HYPHA DISCOVERY LIMITED, Abingdon (GB)
PCT Filed Nov. 26, 2019, PCT No. PCT/GB2019/053337
§ 371(c)(1), (2) Date May 25, 2021,
PCT Pub. No. WO2020/109776, PCT Pub. Date Jun. 4, 2020.
Claims priority of application No. 1819209 (GB), filed on Nov. 26, 2018.
Prior Publication US 2022/0025416 A1, Jan. 27, 2022
Int. Cl. C12P 13/00 (2006.01); C12N 9/02 (2006.01); C12P 7/42 (2006.01); C12P 17/16 (2006.01); C12P 17/18 (2006.01)
CPC C12P 13/005 (2013.01) [C12N 9/0077 (2013.01); C12P 7/42 (2013.01); C12P 17/165 (2013.01); C12P 17/167 (2013.01); C12P 17/182 (2013.01)] 22 Claims
 
1. An enzyme having cytochrome P450 activity and having at least 90% identity to a cytochrome P450 enzyme comprising SEQ ID NO: 29 and comprising at least two of:
(a) a tryptophan residue at the position corresponding to position 63 of SEQ ID NO: 29;
(b) a tyrosine residue at the position corresponding to position 74 of SEQ ID NO: 29; and
(c) an alanine residue at the position corresponding to position 171 of SEQ ID NO: 29.