	US 11,891,629 B2
	Propagator cells and methods for propagating phage, in particular for delivering CRISPR-Cas components via probiotic organisms
Jasper Clube, London (GB)
Assigned to SNIPR TECHNOLOGIES LIMITED, Peterborough (GB)
Appl. No. 16/637,656
Filed by SNIPR Technologies Limited, Peterborough (GB)
PCT Filed Aug. 8, 2018, PCT No. PCT/EP2018/071454 § 371(c)(1), (2) Date Feb. 7, 2020, PCT Pub. No. WO2019/030257, PCT Pub. Date Feb. 14, 2019.
Claims priority of application No. 1712733 (GB), filed on Aug. 8, 2017.
Prior Publication US 2021/0230559 A1, Jul. 29, 2021
Int. Cl. C12N 7/00 (2006.01); A61K 35/74 (2015.01)

CPC C12N 7/00 (2013.01) [A61K 35/74 (2013.01); C12N 2310/20 (2017.05); C12N 2795/00023 (2013.01); C12N 2795/00032 (2013.01); C12N 2795/00052 (2013.01); C12N 2800/80 (2013.01)]

21 Claims

1. A method of producing a population of phages, wherein the phages are of a first type capable of infecting host cells of a first bacterial species or strain by binding a cell-surface receptor comprised by bacteria of the first species or strain, the method comprising:

(a) providing a population of second bacterial cells comprising the receptor on the surface of the second cells, wherein the second cells are of a second species or strain, wherein the second species or strain is different from the first species or strain;

(b) infecting the second cells with the phages of the first type; and

wherein the phages comprise a nucleotide sequence encoding crRNAs that are operable with a Cas in bacteria of the host cell strain or species to form an active CRISPR/Cas system that is capable of targeting one or more protospacer nucleotide sequences, wherein each target sequence is comprised by the genome of the host cells, whereby the crRNAs guide the Cas in host cells to modify the target sequence, thereby killing host cells or reducing host cell population growth; and

wherein the genome of each second bacterial cell does not comprise the target sequence.