US 11,891,627 B2
Mechanical apparatus and method for isolating stromal vascular fraction
Nir Shani, Ramat Hasharon (IL); Eyal Gur, Tel-Aviv (IL); Inna Solodeev, Ramat Gan (IL); and Anna Aronovich, Tel Aviv (IL)
Assigned to THE MEDICAL RESEARCH, INFRASTRUCTURE, AND HEALTH SERVICES FUND OF THE TEL AVIV MEDICAL CENTER, Tel Aviv (IL)
Appl. No. 15/579,802
Filed by The Medical Research, Infrastructure, and Health Services Fund of the Tel Aviv Medical Center, Tel Aviv (IL)
PCT Filed Jun. 9, 2016, PCT No. PCT/IL2016/050609
§ 371(c)(1), (2) Date Dec. 5, 2017,
PCT Pub. No. WO2016/199149, PCT Pub. Date Dec. 15, 2016.
Claims priority of provisional application 62/173,542, filed on Jun. 10, 2015.
Prior Publication US 2018/0155691 A1, Jun. 7, 2018
Int. Cl. C12N 5/0775 (2010.01); C12M 1/33 (2006.01); C12N 5/077 (2010.01); C12M 1/00 (2006.01); B01L 3/00 (2006.01); A61M 1/00 (2006.01)
CPC C12N 5/0667 (2013.01) [A61M 1/80 (2021.05); A61M 1/893 (2021.05); B01L 3/502 (2013.01); C12M 45/02 (2013.01); C12M 47/04 (2013.01); C12N 5/0653 (2013.01); A61M 2202/08 (2013.01); B01L 2300/0681 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0478 (2013.01); C12N 2527/00 (2013.01)] 23 Claims
 
1. A method for enriching a cell population from a mammalian adipose tissue, the method comprising:
aspirating adipose tissue into a container assembly using an aspiration tube having a proximal end detachably connected to the container assembly and a distal end inserted into at least one cavity of a mammalian body;
electrically actuating a mechanical tissue disruption device, while the mechanical tissue disruption device is positioned within the container assembly, with an electrical actuator that is detachably connected to the mechanical tissue disruption device for said electrically actuating, wherein the mechanical tissue disruption device comprises at least one rotatable blade that rotates, when the mechanical tissue disruption device is electrically actuated, to disrupt/rupture the adipose tissue in the container assembly to obtain a cell suspension, wherein the disruption does not utilize an enzyme;
optionally washing the suspension of cells within the container assembly; and
transferring the container assembly, with the suspension of cells being within the container assembly, into a centrifuge and centrifuging the container assembly to thereby obtain a cell pellet from the suspension of cells within the container assembly, and extracting the cell pellet from within the container assembly, and removing the container assembly from the centrifuge;
to thereby obtain an enriched cell population.