CPC C12N 5/0647 (2013.01) [A61K 35/28 (2013.01); A61K 38/193 (2013.01); C12N 15/86 (2013.01); C07K 14/495 (2013.01); C12N 2500/02 (2013.01); C12N 2500/38 (2013.01); C12N 2500/62 (2013.01); C12N 2500/99 (2013.01); C12N 2501/065 (2013.01); C12N 2501/115 (2013.01); C12N 2501/125 (2013.01); C12N 2501/145 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/2303 (2013.01); C12N 2501/2312 (2013.01); C12N 2501/26 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2510/02 (2013.01)] | 19 Claims |
1. A method of enriching a population of cells with hematopoietic stem cells ex vivo, said method comprising contacting a population of cells with one or more agents that together exhibit the activities of:
a. modulation of histone methylation;
b. inhibition of TGFβ signaling;
and
c. modulation of histone acetylation,
wherein the one or more agents are present in amounts that are sufficient to produce a population of cells enriched with hematopoietic stem cells.
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