US 11,891,625 B2
Methods and compositions relating to hematopoietic stem cell expansion, enrichment, and maintenance
Derrick J. Rossi, Newton, MA (US); Wataru Ebina, Boston, MA (US); Morag Stewart, Boston, MA (US); Paula Gutierrez-Martinez, Brookline, MA (US); Lee L Rubin, Wellesley, MA (US); and Lance Davidow, Lexington, MA (US)
Assigned to CHILDREN'S MEDICAL CENTER CORPORATION; and PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Filed by CHILDREN'S MEDICAL CENTER CORPORATION, Boston, MA (US); and PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
Filed on Apr. 21, 2020, as Appl. No. 16/854,190.
Application 16/854,190 is a continuation of application No. 15/738,638, granted, now 10,669,528, previously published as PCT/US2016/039303, filed on Jun. 24, 2016.
Claims priority of provisional application 62/207,136, filed on Aug. 19, 2015.
Claims priority of provisional application 62/184,599, filed on Jun. 25, 2015.
Prior Publication US 2020/0248143 A1, Aug. 6, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/0789 (2010.01); A61K 35/28 (2015.01); A61K 38/19 (2006.01); C12N 15/86 (2006.01); C07K 14/495 (2006.01)
CPC C12N 5/0647 (2013.01) [A61K 35/28 (2013.01); A61K 38/193 (2013.01); C12N 15/86 (2013.01); C07K 14/495 (2013.01); C12N 2500/02 (2013.01); C12N 2500/38 (2013.01); C12N 2500/62 (2013.01); C12N 2500/99 (2013.01); C12N 2501/065 (2013.01); C12N 2501/115 (2013.01); C12N 2501/125 (2013.01); C12N 2501/145 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/2303 (2013.01); C12N 2501/2312 (2013.01); C12N 2501/26 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2510/02 (2013.01)] 19 Claims
 
1. A method of enriching a population of cells with hematopoietic stem cells ex vivo, said method comprising contacting a population of cells with one or more agents that together exhibit the activities of:
a. modulation of histone methylation;
b. inhibition of TGFβ signaling;
and
c. modulation of histone acetylation,
wherein the one or more agents are present in amounts that are sufficient to produce a population of cells enriched with hematopoietic stem cells.