CPC C12N 5/0635 (2013.01) [C12N 2500/30 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2502/1114 (2013.01); C12N 2502/1157 (2013.01); C12N 2502/1185 (2013.01)] | 8 Claims |
1. A method for producing a thymocyte supernatant comprising the following steps:
co-cultivating thymocytes and macrophages at a cell ratio of 0.5:1.2 to 0.5:2 in the presence of phorbol-12-myristate-13-acetate and phytohemagglutinin M for up to 60 hours, and
separating the co-cultivation medium from the cells and thereby producing the thymocyte supernatant.
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