US 11,891,624 B2
Method for the production of thymocyte supernatant
Sonja Offner, Penzberg (DE); Markus Horvath, Penzberg (DE); and Ingried Saam, Bernried (DE)
Assigned to Hoffmann-La Roche Inc., Little Falls, NJ (US)
Filed by Hoffmann-La Roche Inc., Little Falls, NJ (US)
Filed on Oct. 2, 2019, as Appl. No. 16/591,351.
Application 16/591,351 is a continuation of application No. PCT/EP2018/062647, filed on May 16, 2018.
Claims priority of application No. 17171903 (EP), filed on May 19, 2017.
Prior Publication US 2020/0165566 A1, May 28, 2020
Int. Cl. C12N 5/0781 (2010.01)
CPC C12N 5/0635 (2013.01) [C12N 2500/30 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2502/1114 (2013.01); C12N 2502/1157 (2013.01); C12N 2502/1185 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method for producing a thymocyte supernatant comprising the following steps:
co-cultivating thymocytes and macrophages at a cell ratio of 0.5:1.2 to 0.5:2 in the presence of phorbol-12-myristate-13-acetate and phytohemagglutinin M for up to 60 hours, and
separating the co-cultivation medium from the cells and thereby producing the thymocyte supernatant.