US 11,891,599 B2
Recovery of nucleic acids from solid supports
Paresh Lakhubhai Patel, Cardiff South Glamorgan (GB); Lin Chen, ShangHai (CN); Melissa Schwandt, Lafayette, CO (US); Koya Reams, Silver Spring, MD (US); and Thomas Hansen, Silver Spring, MD (US)
Assigned to QIAGEN Healthcare Biotechnologies Systems GmbH, Hilden (DE)
Appl. No. 16/615,645
Filed by QIAGEN Healthcare Biotechnologies Systems GmbH, Hilden (DE)
PCT Filed May 18, 2018, PCT No. PCT/EP2018/063234
§ 371(c)(1), (2) Date Nov. 21, 2019,
PCT Pub. No. WO2018/215375, PCT Pub. Date Nov. 29, 2018.
Application 16/615,645 is a continuation of application No. PCT/CN2017/085296, filed on May 22, 2017.
Claims priority of provisional application 62/536,031, filed on Jul. 24, 2017.
Prior Publication US 2021/0214716 A1, Jul. 15, 2021
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6806 (2018.01)
CPC C12N 15/1006 (2013.01) [C12Q 1/6806 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for recovering nucleic acids from a solid support, the method comprising:
(a) combining a biological sample with a region of a solid support, wherein the region is absorbent and impregnated with a chaotropic agent;
(b) washing the region in a washing buffer solution, wherein the washing buffer solution is an alkaline solution;
(c) simultaneously heating and agitating the region in a further alkaline buffer solution;
(d) separating the region from the further alkaline buffer solution without centrifugation, wherein the separating of the region from the further alkaline buffer solution is performed by disposing the region in a porous container above the remaining further alkaline buffer solution;
(e) extracting at least a portion of any remaining further alkaline buffer solution from the region to provide an extracted alkaline buffer solution, wherein the separating and the extracting are performed using different methods;
(f) combining the further alkaline buffer solution from (d) and the extracted alkaline buffer solution portion; and
(g) subsequently processing the combined buffer solutions in order to amplify any nucleic acids in said combined solution.