	US 11,891,451 B2
	Glycosylation of variable immunoglobulin domains
Nico Callewaert, Nevele (BE); Bram Laukens, Ghent (BE); Loes Van Schie, Ghent (BE); Wander Van Breedam, Antwerp (BE); and Wim Nerinckx, Astene (BE)
Assigned to VIB VZW, Ghent (BE); and Universiteit Gent, Ghent (BE)
Appl. No. 16/612,476
Filed by VIB VZW, Ghent (BE); and UNIVERSITEIT GENT, Ghent (BE)
PCT Filed May 9, 2018, PCT No. PCT/EP2018/062154 § 371(c)(1), (2) Date Nov. 11, 2019, PCT Pub. No. WO2018/206734, PCT Pub. Date Nov. 15, 2018.
Claims priority of application No. 17170661 (EP), filed on May 11, 2017.
Prior Publication US 2020/0148790 A1, May 14, 2020
Int. Cl. C07K 16/40 (2006.01); A61K 47/68 (2017.01)

CPC C07K 16/40 (2013.01) [A61K 47/6871 (2017.08); C07K 2317/22 (2013.01); C07K 2317/41 (2013.01); C07K 2317/565 (2013.01); C07K 2317/567 (2013.01); C07K 2317/569 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01)]

13 Claims

1. A nucleotide sequence encoding a polypeptide, wherein the polypeptide comprises:

an immunoglobulin single variable domain (ISVD) derived from a camelid heavy chain antibody, wherein the ISVD comprises an amino acid sequence that comprises 4 framework regions (FR) and 3 complementarity determining regions (CDR) according to the following formula (1):

FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 (1),

wherein the ISVD further comprises a N-glycosylation acceptor site present on an amino acid selected from amino acids 83 to 88 of the ISVD according to the AHo numbering convention.