	US 11,891,430 B2
	Multistep final filtration
Roberto Falkenstein, Munich (DE); and Klaus Schwendner, Weilheim (DE)
Assigned to Hoffmann-La Roche Inc., Little Falls, NJ (US)
Filed by Hoffmann-La Roche Inc., Little Falls, NJ (US)
Filed on Oct. 22, 2019, as Appl. No. 16/660,635.
Application 16/660,635 is a continuation of application No. 15/444,018, filed on Feb. 27, 2017, abandoned.
Application 15/444,018 is a continuation of application No. 13/394,766, abandoned, previously published as PCT/EP2010/064487, filed on Sep. 29, 2010.
Claims priority of application No. 09012460 (EP), filed on Oct. 1, 2009.
Prior Publication US 2020/0291097 A1, Sep. 17, 2020
Int. Cl. C07K 16/06 (2006.01); C07K 16/28 (2006.01); C07K 16/32 (2006.01); C07K 1/16 (2006.01); C07K 1/34 (2006.01); C07K 1/36 (2006.01)

CPC C07K 16/065 (2013.01) [C07K 16/2866 (2013.01); C07K 16/32 (2013.01)]

16 Claims

1. A method for producing an immunoglobulin solution comprising

a) providing an immunoglobulin solution with a concentration of at least 100 g/l, and

b) applying the immunoglobulin solution to a combination of a first and second filter unit, whereby the first filter unit comprises a pre-filter with a pore size of 3.0 μm and a main-filter with a pore size of 0.8 μm and the second filter unit comprises a pre-filter with a pore size of 0.45 μm and a main-filter with a pore size of 0.22 μm with a pressure of from 0.1 to 4.0 bar, and thereby producing an immunoglobulin solution.