US 11,891,414 B2
Sample plates for buffer exchange and methods of manufacture
David Wesley Wyrick, Pleasanton, CA (US); Stuart Kazumi Morita, Sunnyvale, CA (US); Lynn Comiskey, San Jose, CA (US); and David Eugene Eberhart, Santa Clara, CA (US)
Assigned to Unchained Labs, Pleasanton, CA (US)
Filed by Unchained Labs, Pleasanton, CA (US)
Filed on Mar. 21, 2018, as Appl. No. 15/927,934.
Claims priority of provisional application 62/474,757, filed on Mar. 22, 2017.
Prior Publication US 2018/0273583 A1, Sep. 27, 2018
Int. Cl. B01L 3/00 (2006.01); C07K 1/34 (2006.01); C07K 16/00 (2006.01); B01D 61/14 (2006.01); B01D 61/02 (2006.01); G01N 1/34 (2006.01); G01N 1/40 (2006.01)
CPC C07K 1/34 (2013.01) [B01D 61/027 (2013.01); B01D 61/145 (2013.01); B01L 3/50255 (2013.01); C07K 16/00 (2013.01); G01N 1/34 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0851 (2013.01); G01N 2001/4088 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A sample plate for automated exchange of a buffer solution from a biological sample comprising:
a plurality of reservoirs, wherein each reservoir of the plurality of reservoirs comprises a side wall, a ring shaped inner ridge, a filter attachment region comprising a thermal seal and an adhesive seal, and a ring shaped channel defined by the side wall and the ring shaped inner ridge, wherein the ring shaped channel comprises the thermal seal and is filled with an adhesive of the adhesive seal; and
a filter having a diameter configured to accommodate a height of the ring shaped inner ridge so that a peripheral edge of the filter may be secured to each reservoir at the filter attachment region,
wherein the filter attachment region maintains fixation of the filter peripheral edge to each reservoir at a pressure differential across the filter of at least about 30 psig.