US 11,891,411 B2
Industrial utilization method for Stevia rebaudiana and stevioside and chlorogenic acid of Stevia rebaudiana
Meili Xu, Hebei (CN); Yunhe Lian, Hebei (CN); Hong Tian, Hebei (CN); Wei Gao, Hebei (CN); and Yachao Xu, Hebei (CN)
Assigned to Chenguang Biotech Group Co., Ltd., Hebei (CN)
Filed by Chenguang Biotech Group Co., Ltd., Hebei (CN)
Filed on Mar. 30, 2021, as Appl. No. 17/216,928.
Application 17/216,928 is a continuation of application No. PCT/CN2019/108619, filed on Sep. 27, 2019.
Claims priority of application No. 201811159791.7 (CN), filed on Sep. 30, 2018.
Prior Publication US 2021/0230204 A1, Jul. 29, 2021
Int. Cl. C07H 15/24 (2006.01); C07C 67/56 (2006.01)
CPC C07H 15/24 (2013.01) [C07C 67/56 (2013.01)] 12 Claims
 
1. An industrial utilization method for extracting Stevia rebaudiana, comprising:
1) Extracting Stevia rebaudiana powder with a high-concentration short-chain alcohol aqueous solution to obtain an extract solution, wherein the short-chain alcohol in the high-concentration short-chain alcohol aqueous solution is selected from methanol, ethanol, propanol or any combination thereof, and a volume fraction of the short-chain alcohol in the short-chain alcohol aqueous solution is 70% to 95%;
2) Removing the short-chain alcohol in the extract solution by vacuum concentration, and subjecting the extract solution to liquid-liquid extraction with an organic solvent, and taking water layer to obtain a low-polar impurity-removed extract solution, wherein the organic solvent is a low-polar organic solvent that is water insoluble; and
3) Adjusting pH of the low-polar impurity-removed extract solution to be alkaline, and passing the extract solution through a stevioside adsorption resin to obtain a stevioside extract.