US 11,890,620 B2
Miniaturized DNA microarray for small-volume sample processing
Kirsty A. McFarland, Melrose, MA (US); Charles A. Lissandrello, Newtonville, MA (US); Andrew P. Magyar, Arlington, MA (US); and Erin Rosenberger, Quincy, MA (US)
Assigned to The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US)
Appl. No. 16/755,505
Filed by The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US)
PCT Filed Oct. 12, 2018, PCT No. PCT/US2018/055600
§ 371(c)(1), (2) Date Apr. 10, 2020,
PCT Pub. No. WO2019/075321, PCT Pub. Date Apr. 18, 2019.
Claims priority of provisional application 62/687,012, filed on Jun. 19, 2018.
Claims priority of provisional application 62/572,264, filed on Oct. 13, 2017.
Prior Publication US 2021/0220823 A1, Jul. 22, 2021
Int. Cl. B01L 3/00 (2006.01); B01L 7/00 (2006.01); B04B 3/00 (2006.01); B04B 5/04 (2006.01); C12Q 1/6837 (2018.01); C12Q 1/6874 (2018.01)
CPC B01L 3/50853 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502746 (2013.01); B01L 7/00 (2013.01); B04B 3/00 (2013.01); B04B 5/0414 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6874 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/16 (2013.01); B01L 2300/044 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/168 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A miniaturized DNA microarray assembly, consisting of:
a) a single high-density array of oligonucleotide probes printed on a single solid substrate, wherein the dimensions of the solid substrate are about 14.0 mm by about 8 mm, or less;
b) an inert enclosure surrounding sides and bottom of the single solid substrate, wherein the enclosure has a fluid inlet port and a fluid outlet/exit port which allows fluid to flow over and contact the oligonucleotide probes of the array; and
c) an optically transparent top surface sealed to the enclosure.