	US 11,890,318 B2
	Methods and compositions for modulating appetite and intake of sodium
Yuki Oka, Pasadena, CA (US); and Sangjun Lee, Pasadena, CA (US)
Assigned to California Institute of Technology, Pasadena, CA (US)
Filed by California Institute of Technology, Pasadena, CA (US)
Filed on Jan. 9, 2020, as Appl. No. 16/738,380.
Claims priority of provisional application 62/792,257, filed on Jan. 14, 2019.
Prior Publication US 2020/0222500 A1, Jul. 16, 2020
Int. Cl. A61K 38/17 (2006.01); C12N 15/86 (2006.01); A61K 9/00 (2006.01)

CPC A61K 38/1709 (2013.01) [A61K 9/0019 (2013.01); C12N 15/86 (2013.01); C12N 2750/14143 (2013.01)]

6 Claims

1. A method of reducing sodium appetite in a subject in need thereof, the method comprising: inhibiting a plurality of prodynorphin (PDYN)-positive neurons in the pre-locus coeruleus (pre-LC^PDYNneurons) of the subject, wherein inhibiting the plurality of pre-LC^PDYNneurons of the subject comprises chemogenetically inhibiting the plurality of pre-LC^PDYNneurons, thereby reducing sodium ingestion of the subject; and wherein sodium ingestion of the subject is reduced within 50 minutes or less relative to a subject wherein the pre-LC^PDYNneurons have not been chemogenetically inhibited, following the chemogenetic inhibition of neurons in the pre-LC that express PDYN.