	US 11,890,230 B2
	Three-dimensional image guided scanning irradiation device for targeted ablation, stimulation, manipulation, molecular delivery and physiological monitoring
Samarendra Mohanty, Southlake, TX (US); Sanghoon Kim, Euless, TX (US); Subrata Batabyal, Arlington, TX (US); and Michael Carlson, Dallas, TX (US)
Assigned to Nanoscope Technologies, LLC, Bedford, TX (US)
Filed by Nanoscope Technologies, LLC, Bedford, TX (US)
Filed on Dec. 7, 2020, as Appl. No. 17/114,126.
Application 17/114,126 is a continuation in part of application No. 16/074,041, filed on Jul. 30, 2018, granted, now 10,857,238.
Claims priority of provisional application 62/295,030, filed on Feb. 13, 2016.
Prior Publication US 2021/0177649 A1, Jun. 17, 2021
Int. Cl. A61N 5/06 (2006.01); A61F 9/008 (2006.01); A61N 5/067 (2006.01); A61B 18/20 (2006.01); A61B 18/00 (2006.01); A61B 17/00 (2006.01); A61M 37/00 (2006.01); A61B 3/135 (2006.01); A61B 3/10 (2006.01)

CPC A61F 9/008 (2013.01) [A61N 5/062 (2013.01); A61N 5/067 (2021.08); A61B 3/102 (2013.01); A61B 3/135 (2013.01); A61B 2017/00154 (2013.01); A61B 2018/00428 (2013.01); A61B 2018/00791 (2013.01); A61B 2018/20553 (2017.05); A61F 2009/00851 (2013.01); A61F 2009/00863 (2013.01); A61F 2009/00891 (2013.01); A61M 2037/0007 (2013.01); A61N 2005/0662 (2013.01)]

21 Claims

1. A device comprising:

a. a laser irradiation sub-assembly configured to generate a laser beam for irradiation of a sample comprising at least one of living tissue, neurons, retina cells, brain cells, heart cells, muscle cells, epithelial cells, endothelial cells, blood and skin cells;

b. a visible light source combinable with the laser beam by a beam combiner and configured to at least one of:

locate the laser beam;

stimulate the sample; and

fixate the sample;

c. an imaging light source sub-assembly which emits a light beam with a selected wavelength in a range from 400 nm to 1600 nm, wherein the selected wavelength is not perturbative to the functioning or structure of the sample;

d. wherein the light beam emitted from the imaging light source sub-assembly is combinable with the laser beam and able to be split by a beam splitter to generate a split combined beam having a first part and second part;

e. wherein the first part of the split combined beam is guided via an optical system comprising at least one mirror, at least one focusing element and at least one lens to irradiate the sample;

f. wherein the at least one mirror and at least one focusing element is actuatable to control the position and size of an irradiation spot on the sample;

g. wherein back-scattered light from the sample is able to pass through the optical system and the beam splitter to a detector;

h. wherein the second part of the split combined beam is able to traverse through an optical sub-assembly comprising a reference arm;

i. wherein optionally, back-reflected light from the reference arm is able to pass through the beam splitter to the detector to interfere with the back-scattered light from the sample;

j. wherein a signal from the detector is processed to obtain at least one of:

a depth-resolved image of the sample;

measurements of physical properties of the sample; and

measurements of physiological properties of the sample;

k. wherein a laser beam irradiation dose and pattern in a targeted region is controllable for performing at least one of ablation, stimulation, molecular delivery and alteration of the sample; and

l. wherein a dose and pattern of the light beam from the visible light source is controllable and able to be synchronized with the imaging light source for mapping functional properties of the sample.