CPC A23J 1/14 (2013.01) [A23C 11/103 (2013.01); A23C 11/106 (2013.01); A23J 1/006 (2013.01); A23J 3/14 (2013.01); A23L 9/24 (2016.08); A23L 11/60 (2021.01); A23L 33/185 (2016.08); C07K 1/145 (2013.01); C07K 1/30 (2013.01); C07K 14/415 (2013.01); C07K 16/00 (2013.01); A23V 2002/00 (2013.01); A23V 2200/00 (2013.01)] | 11 Claims |
1. A method for obtaining a refined protein component from a non-animal natural and/or modified non-animal natural source comprising the steps of:
a) obtaining a protein preparation from the non-animal natural or modified non-animal natural source;
b) washing the protein preparation at a wash pH;
c) aqueously extracting the protein preparation at an extraction pH to obtain an aqueous protein solution;
d) separating the aqueous protein solution from non-aqueous components;
e) heating the aqueous protein solution to a temperature of about 60° C.;
f) adding a calcium salt suitable for coagulating protein and adjusting the aqueous protein solution to a precipitation pH between about 6 and about 3 to precipitate protein and obtain a protein precipitate;
g) separating the protein precipitate from non-precipitated components; and
h) washing the protein precipitate to obtain the refined protein component;
wherein step (f) is the only protein precipitation step in the method.
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