US 11,889,847 B2
Method for producing a coffee extract employing enzymes having beta-1,3-galactanase activity
Nikolaj Spodsberg, Bagsvaerd (DK); Kristian Bertel Roemer M Krogh, Bagsvaerd (DK); Rune Nygaard Monrad, Hilleroed (DK); Jens Ekloef, Copenhagen (DK); Louise Rasmussen, Helsinge (DK); Gitte Budolfsen Lynglev, Frederiksberg (DK); and Laure Coulomb, Toulouse (FR)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Appl. No. 14/909,576
Filed by Novozymes A/S, Bagsvaerd (DK)
PCT Filed Aug. 15, 2014, PCT No. PCT/EP2014/067504
§ 371(c)(1), (2) Date Feb. 2, 2016,
PCT Pub. No. WO2015/022428, PCT Pub. Date Feb. 19, 2015.
Claims priority of application No. 13180578 (EP), filed on Aug. 15, 2013.
Prior Publication US 2016/0249635 A1, Sep. 1, 2016
Int. Cl. A23F 5/24 (2006.01); A23F 5/36 (2006.01); C12N 9/24 (2006.01)
CPC A23F 5/246 (2013.01) [A23F 5/36 (2013.01); C12N 9/2402 (2013.01); C12Y 302/01145 (2013.01); C12Y 302/01181 (2015.07)] 10 Claims
 
1. A method for producing a coffee extract which comprises at least 20% total galactose based on the total weight of soluble coffee solids, comprising the steps:
(a) adding water, an endo-β-1,3-galactanase (EC 3.2.1.181), and a β-mannanase to roasted and ground coffee beans, wherein the endo-β-1,3-galactanase is added at a concentration of at least 0.001 g/kg coffee beans and a β-mannanase is added in an amount to produce an increased yield of solubilised coffee solids in the coffee extract which is greater than additive as compared to the increased yield of solubilised coffee solids obtained by incubation with each enzyme alone;
(b) incubating to make an aqueous coffee extract; and
(c) separating the coffee extract from the coffee beans;
wherein the coffee extract comprises at least 20% total galactose, based on the total weight of soluble coffee solids.