US 11,867,685 B2
Olfactory receptor involved in the perception of musk fragrance and the use thereof
Sandra Huysseune, Brussels (BE); Alex Veithen, Genappe (BE); and Yannick Quesnel, Wavre (BE)
Assigned to CHEMCOM S.A., Brussels (BE)
Appl. No. 16/770,457
Filed by CHEMCOM S.A., Brussels (BE)
PCT Filed Dec. 5, 2018, PCT No. PCT/EP2018/083570
§ 371(c)(1), (2) Date Jun. 5, 2020,
PCT Pub. No. WO2019/110630, PCT Pub. Date Jun. 13, 2019.
Claims priority of application No. 17205402 (EP), filed on Dec. 5, 2017.
Prior Publication US 2021/0164967 A1, Jun. 3, 2021
Int. Cl. G01N 33/50 (2006.01)
CPC G01N 33/5008 (2013.01) 19 Claims
 
1. A method for identifying an agent or a sample comprising one or more agent(s) that interfere with the binding between an OR5A2 receptor and musk compounds, said method comprising:
a) contacting a chimeric receptor with said agent or sample, wherein said chimeric receptor comprises a central region of the OR5A2 receptor, encompassing the transmembrane domains 2 to 7 having the amino acid sequence of SEQ ID NO: 11, or a polypeptide sequence having at least 95% sequence identity to SEQ ID NO: 11, with the proviso that said polypeptide has a proline at position 114 of said polypeptide sequence having at least 95% sequence identity to SEQ ID NO: 11 and,
wherein the central region is fused at its N-terminus to the N-terminal extracellular moiety, the transmembrane domain 1 and the intracellular loop 1 of a G protein-coupled receptor, and which is fused at its C-terminus to the intracellular C-terminal end of a G protein-coupled receptor,
wherein the chimeric receptor is capable of binding musk compounds selected from the group consisting of: nitromusks, macrocyclic musks, polycyclic musks, and linear musks;
b) measuring a signaling activity of the chimeric receptor in the presence of said agent or sample; and
c) comparing the activity measured in the presence of said agent or sample to the activity measured in a reaction in which the chimeric receptor is contacted with one or more musk compound(s) at its/their EC50, wherein said agent or sample is identified as an agent or a sample, that modulates the activity of said chimeric receptor as defined herein when the amount of the activity measured in the presence of the agent or sample is at least 10% of the amount induced by said musk compound(s) at its/their EC50.