US 11,867,672 B2
Flow cell with one or more barrier features
Tarun Khurana, Fremont, CA (US); Ali Agah, Menlo Park, CA (US); Aathavan Karunakaran, Berkeley, CA (US); Stanley Hong, Palo Alto, CA (US); Merek Siu, Alameda, CA (US); Arvin Emadi, San Jose, CA (US); and Craig Ciesla, Mountain View, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Feb. 9, 2023, as Appl. No. 18/107,752.
Application 18/107,752 is a continuation of application No. 17/846,538, filed on Jun. 22, 2022, granted, now 11,604,175.
Application 17/846,538 is a continuation of application No. 17/479,342, filed on Sep. 20, 2021, granted, now 11,402,358, issued on Aug. 2, 2022.
Application 17/479,342 is a continuation of application No. 17/254,466, granted, now 11,143,638, issued on Oct. 12, 2021, previously published as PCT/US2020/034518, filed on May 26, 2020.
Claims priority of provisional application 62/855,654, filed on May 31, 2019.
Claims priority of provisional application 62/855,662, filed on May 31, 2019.
Prior Publication US 2023/0184725 A1, Jun. 15, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 30/74 (2006.01); C12Q 1/6874 (2018.01)
CPC G01N 30/74 (2013.01) [C12Q 1/6874 (2013.01); G01N 2030/746 (2013.01)] 13 Claims
OG exemplary drawing
 
1. An apparatus, comprising:
(a) a flow cell body defining one or more flow channels and a plurality of wells, each flow channel of the one or more flow channels to receive a flow of fluid, each well of the plurality of wells being fluidically coupled with the corresponding flow channel of the one or more flow channels, each well of the plurality of wells defining a corresponding depth;
(b) a plurality of electrodes, each electrode of the plurality of electrodes being positioned in a corresponding well of the plurality of wells, the plurality of electrodes to effect writing of polynucleotides in the corresponding wells of the plurality of wells; and
(c) a temperature gradient generator, the temperature gradient generator to provide a temperature profile that varies across the depth of each well of the plurality of wells.